摘要
目的探讨妊娠高血压疾病患者中可溶性血管内皮生长因子受体-1mRNA在胎盘组织中的表达。方法23例妊娠高血压疾病孕妇(其中妊娠期高血压7例,轻度子痫前期3例,重度子痫前期9例,子痫4例)为妊娠高血压疾病组,足月妊娠血压正常孕妇作为对照组。应用半定量逆转录-聚合酶链反应(RT-PCR)技术检测两组孕妇分娩后胎盘组织中sF lt-1的表达强度。结果妊娠高血压疾病组sF lt-1mRNA(1.45±1.47),与对照组(1.04±0.69)比较,差异无显著性(P>0.05)。子痫组(2.99±2.47),明显高于对照组(P<0.01)。结论sF lt-1是维持正常妊娠所必需的因子。它表达水平的高低与妊娠高血压的发病相关,在子痫患者它的表达明显增高。
Objective ： The purpose of this study is to determine the mRNA expression of sFlt - 1 in placenta with hypertensive disorder complicating pregnancy（HDCP）. Methods： Twenty three women with HDCP which within seven gestational hypertension, twelve preeclampsia, four eclampsia were enrolled in this study. Twenty healthy pregnancies were recruited as control group. Placenta tissues were ebtained from HDCP and healthy control groups for analysis of sFlt - 1 mRNA expression by using quantitative reverse transcriptase - polymerase chain reaction （ RT - PCR）. Results ： The expression of sFlt - 1 mRNA were not different between HDCP group 1.45 ± 1.47 and control group 1.04 ±0. 69 （ P 〉0. 05）. But the placentas of eclampsia group expressed sFlt - 1 mRNA levels at significantly higher levels 2.99 ± 2. 47 than that of control group （ P 〈 0. 01 ）. Conclusion： The results suggest that sFlt - 1 is necessary for pregnancy. The expression of sFlt - 1 mRNA is increased in women with eclampsia .
出处
《中国优生与遗传杂志》
2006年第3期22-23,共2页
Chinese Journal of Birth Health & Heredity
