摘要
目的探讨内毒素(LPS)致幼鼠肠损伤及谷氨酰胺(glutam ine G ln)的保护作用。方法10日龄W istar幼鼠共72只,分为A组:生理盐水组,B组:内毒素组,C组:内毒素+谷氨酰胺组。以EcoliO55:B5(1m l/kg)、生理盐水(1m l/kg)谷氨酰胺(10m l/kg)腹腔注射,给药后2、6、24h处死动物,留取肠组织,免疫组化检测肠组织肿瘤坏死因子-α(tumor necrosis factor-,αTNF-α)蛋白含量,生化法检测肠组织MDA含量,同时作电镜观测肠组织超微结构变化。结果B组TNF-α积分光密度、MDA含量明显高于A组,以LPS作用后6h达高峰(37247.64±3168.80 vs 6191.02±482.32,P<0.01;7.601±1.144 vs 5.014±0.741,P<0.01)。C组TNF-α、MDA含量较B组明显降低,其中各时间点TNF-α较B组下降,但仍高于A组,P<0.01;24h C组MDA含量较B组下降,P<0.01;但较A组无差异,P>0.05。B组肠组织超微结构变化明显,C组肠组织超微结构变化较B组不同程度减轻。结论LPS可致幼鼠肠损伤,G ln对肠损伤有保护作用。
Objectives: To study the effects of LPS to intestines of infant rats and testify the protective effects of glutamine. Methods: 10 -day -old Wistar infant rats were divided by groups A, B, C. Group A were served as the normal control group and injected with normal saline (1ml/kg) intraperioneally. Group B and C were treated with LPS (1ml/kg) and LPS (1ml/kg) ± Gln (10ml/kg) intraperioneal respectively. Followed by LPS administration, intestinal tissues were collected at 2, 6, and 24h to examine TNF - α expression, MDA productions and intestinal histological changes. Results : It can be seen the TNF - α expression in epithelial cells of intestines. The IODT of TNF - α and MDA contents were significantly increased in group B and reached the maximum 6h after LPS administration (37247.64 ± 3168. 80 vs 6191.02 ±482. 32, P 〈 0.01 ; 7. 601 ± 1. 144 vs 5. 014 ± 0. 741, P 〈 0. 01 ). Contrasted with group B, The expression of TNF - α of group C were decreased and still sustained high level at every time, P 〈 0.01. Whereas the contents of MDA were decreased than group B ( P 〈0. 01) but had no discrepancy with group A ( P 〉0.05) at 24h group. The ultra structure of intestinal tissues had obvious changes in group B, which improved in group C. Conclusion: LPS can induce the intestinal injuries in infant rats and glutamine have marked protectively effects.
出处
《中国优生与遗传杂志》
2006年第3期30-32,共3页
Chinese Journal of Birth Health & Heredity
关键词
肠
内毒素
谷氨酰胺
肿瘤坏死因子
丙二醛
Intestine
Endotoxin
Glutamine
Tumor necrosis factor - α
Malondialdehyde