高通量筛选法对普洱茶降血糖血脂作用的研究

Study on Blood Glucose-Depressing and Blood Lipid-Depressing Function of Pu’er Tea by High Throughput Screening Method

利用高通量筛选法寻找普洱茶降血糖血脂作用的活性成分的研究。实验以SMMC-7721细胞为模型,确定普洱茶各样品的合适筛选浓度为50μg/ml,并以此浓度对激活PPARα、PPARδ、PPARγ受体的活性进行筛选。结果发现,普洱茶醇提物对PPARδ受体有激活作用,有潜在的降血糖和降血脂作用的可能。

Discovering the active substances of Pu＇er Tea on blood glucose depressing and blood lipid-depressing function was studied by high throughput screening method. In this experiment, SMMC-7721 cells were used as the model, the screening concentration of samples was established at to 50 μg/ml, and the activity of Pu＇er Tea on activing PPARα、PPARδ, PPARγ was screening. The result showed that Pu＇er Tea ethanol extract can active PPAR 5 and may be have the function of lowering blood glucose and blood lipid depressing function.