摘要
目的 探讨阿魏酸钠对糖尿病大鼠肾组织TGF-β1和Smad4表达的影响。方法 制作链脲佐菌素诱导的Wistar糖尿病大鼠模型，将其随机分为3组（对照组、糖尿病非治疗组、糖尿病阿魏酸钠治疗组），给药8周后将其处死，检测各组大鼠的平均动脉压、血糖、肾功能指标、24h尿蛋白，并检测TGF-β1，及Smad4mRNA表达水平和蛋白定位的表达。结果与对照组相比，糖尿病大鼠平均动脉压、血糖、肾功能指标、24h尿蛋白均升高；肾小球细胞外基质（ECM）明显增多、系膜区扩大（PAS染色）；TGF-β1及Smad4mRNA水平上升；TGF-β1，及Smad4蛋白的表达明显增加。阿魏酸钠治疗后上述指标明显下降。结论 糖尿病肾病患者，TGF-β1／Smad通路被激活，使用阿魏酸钠治疗可以延缓肾脏损害的发生。
Objective To investigate the effect of Sodium ferulate on expression of TGF-β1 and Smad4 in kidney tissiue of diabetic rats. Method Healthy male Wistar rats were divided into three groups randomly, normal control group, strepotozocln induced diabetic rats group and strepotozocin induced diabetic rats treated with Sodium ferulate. The rats were sacrificed after treated for eight weeks. Mean aterial pressure, plasma glucose, BUN, SCr, twenty-four hours urinary protein of were observed. The level of TGF-β1 and Smad4 mRNA were examined by RT-PCR. The sites and level of TGF-β1 and Smad4 protein expression were examined by immunohistochemistry staining. Results Compared to normal control group, mean aterial pressure, plasma glucose , BUN, SCr, urinary protein of twenty-four hours were significantly increased in diabetic rats group, and the group was also associated with marked glomerular extracellular matrix accumulation and mesangial expansion（PAS stai- ning）. The level of TGF-β1 and Smad4 mRNA were up-regulated as well as the expression of TGF-β1 and Smad4 protein. Sodium ferulate administration made all changes much better. Conclusions TGF-β1 Smad signaling is activated in diabetic nephropathy, Sodium ferulate administration can attenuate renal damage in diabetes.
出处
《临床肾脏病杂志》
2006年第1期7-10,共4页
Journal Of Clinical Nephrology
