摘要
目的研究血红素氧合酶(HO)在门静脉高压症患者脾动脉的表达,探讨血红素氧合酶-内源性一氧化碳系统(HO-CO)在门静脉高压症演进过程中的作用。方法收集门静脉高压症并行择期脾切除加贲门周围血管离断术患者18例,同期外伤性脾破裂行脾切除术患者12例为对照,取脾动脉组织,应用半定量逆转录聚合酶链反应(RT-PCR)检测HO-1、HO-2 mRNA表达,Western blot检测其蛋白表达。结果HO-1 mRNA和蛋白表达的吸光度比值在对照组为(0.03±0.00)、(0.04±0.01),均明显低于门静脉高压组(0.81±0.12)、(1.56±0.25),差异均有极显著性意义(P<0.01);HO-2 mRNA和蛋白表达在对照组为(0.64±0.12)、(0.84±0.14),在门静脉高压组为(0.58±0.09)、(0.92±0.12),差异均无显著性意义(P>0.05)。HO-1表达与肝功能分级相关,肝功能Ⅱ级患者其脾动脉HO-1 mRNA和蛋白表达均明显高于肝功能Ⅰ级患者(P<0.01);HO-2表达与肝功能分级无相关关系(P>0.05)。结论HO-CO系统,尤其是HO-1在门静脉高压症演进过程中起重要作用。
Objective To study the role of hemeoxygenase-carbon monoxide system （HO-CO） in the progression of portal hypertension by detecting the expression of HO in splenic arteries of portal hypertensive patients. Methods Eighteen portal hypertensive patients subject to elective splenectomy plus Hassab operation and 12 cases of traumatic rupture of spleen undergoing splenectomy as control group were studied. Semi-quantitative RT-polymerase chain reaction and Western blot were used to detect the expression of HO-1 and HO-2 mRNA and proteins respectively in the splenic arteries. Results The mRNA and protein expression of HO-1 in control group was（0.03±0.00） and （0.04±0.01） respectively, significantly lower than that in portal hypertension group [（0. 81±0.12） and （1.56±0.25） respectively, both P〈0.01]. ThemRNAand protein expression of HO-2 in control group and portal hypertension group was （0.61±0. 12） vs （0.58±0.09）. （0.84±0. 14） vs （0.92±0.12）. respectively, with the difference being not significant （both P〈0.05）. In the patients with grade Ⅱ. the mRNA and protein expression of HO-1 was significantly higher than in those with grade Ⅰ （both P（0.01）. Conclusion HO-CO system, especially HO-1. plays an important role in the progression of portal hypertension.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2006年第1期56-58,62,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目(No.30170920)
