E-选择素S128R基因多态性与吸烟在冠心病发生中的交互作用

Interaction of E-selectin S128R Gene Polymorphism and Smoking on Coronary Heart Disease

目的探讨E-选择素S128R基因多态性与吸烟在汉族人群冠心病病理变化过程中的交互作用。方法采用病例-对照研究方法对197例冠心病(CHD)患者和从正常人群中抽取的213例对照人群进行调查。聚合酶链反应-限制性片段长度多态性技术检测E-选择素S128R基因型在冠心病组和对照组中的基因频率分布。问卷调查吸烟习惯在冠心病组和对照组中的分布频率。结果冠心病组SR+RR基因型频率(17.77%)显著高于对照组(5.16%),P<0.05。冠心病组E-选择素血清水平[(37.8±7.3)μg/L]与对照组[(27.5±8.1)μg/L]比较差异有极显著性意义(P<0.01);冠心病组中SR+RR基因型携带者的E-选择素血清水平[(43.3±7.9)μg/L]显著高于SS基因型者[(34.2±7.5)μg/L,P<0.01]。调整年龄、性别、血压、体重指数后,E-选择素S128R基因多态性与吸烟对冠心病发生交互作用有显著意义的OR值分别为:SR+RR-曾吸烟1.56,SR+RR-仍吸烟4.52。结论SR+RR基因型携带者经常大量吸烟,其患冠心病的危险性显著增加。

Objective To investigate the interaction between the E selectin S128R gene polymorphism and smoking on coronary heart disease （CHD） in pathogenesis of coronary atherosclerosis in Hans of Chinese. Methods 197 patients with CHD and 213 volunteers without documented evidence of CHD were subjected to a case-control study. E-selectin S128R polymorphism in the CHD group and control group respectively were detected by using the method of polymerase chain reaction-restriction fragment length polymorphisms. Results For the E-selectin S128R gene polymorphism, the frequencies of SR＋RR genotype were 17.77 % in the CHD group, significantly higher than those in the control group （5.16%, P〈0.05）. The levels of E-selectin in the CHD group[ （37.8±7.3） μg/L] were significantly higher than those in the control group [（27.5±8.1） μg/ L , P〈0. 01]. The levels of E-selectin E（43. 3±7. 9） μg/l.] in the SR4-RR genotype in the CHD group were significantly higher than those in the SS-genotype [（34. 2±7. 5） μg/L. P〈001]. After adjustment of confounding variables, such as age, sex, blood pressure, and body mass index, the binary logistic analysis showed a significant gene-environment interaction. The OR values were： SR＋RR-stopped smoking （1.56）, SR＋RR-smoking （4.52） respectively. Conclusion The carrier of SR＋RR alleles would have higher risk suffering from CHD if they are smoking heavily.