顺铂对人腺样囊性癌细胞端粒酶基因表达的影响

The effects of cisplation on telomerase activity of human adenoid cystic carcinoma

目的:探讨顺铂抗人腺样囊性癌细胞(ACC-2)的作用机制。方法:采用MTT比色法研究顺铂对人腺样囊性癌(ACC-2)细胞和涎腺细胞(HSG)的相对选择性,应用原位杂交法检测人腺样囊性癌细胞端粒酶基因表达的变化。结果:顺铂对人腺样囊性癌细胞的抑制率高达98.4%,明显高于同期培养的涎腺细胞(HSG)(67.9%),具有显著性差异(P<0.01)。人腺样囊性癌细胞(ACC-2)的端粒酶阳性细胞为88.49%,顺铂组为25.62%,具有显著性差异(P<0.01)。结论:顺铂对人腺样囊性癌细胞(ACC-2)端粒酶基因的表达具有明显的抑制作用。

Objective： To investigate the effects of cisplatin on telomerase activity of human adenoid cystic carcinoma and to find out the possible mechanisms of it＇s anticancer action. Method：ACC-2 and HSG were treated with cisplatin of different concentration for 72 h. Then, the proliferation of the ceils was measured by MTT method, telomerase activity was measured by telomeric repeat amplification protocol with ELISA（TRAP- ELISA）. Result： The inhibition rate of human adenoid cystic carcinoma cells by cisplatin was 98.4% which is significantly higher than that of HSG（67.9% ） （P〈 0.01 ）. There is also a significant difference （ P 〈 0.01 ） between t dcxnerase positive cells of ACC-2 （88.49 % ） and HSG （26.62%）. Condusion：Cisplatin could obviously inhibit the telomerase activity of ACC-2, which maybe the important mechanisms of cisplatin anticancer action.