利用RAPD技术寻找大豆抗孢囊线虫4号小种标记初报

PRILIMINARY STUDY ON IDENTIFICATION OF RAPD MARKER ASSOCIATED WITH RESISTANCE TO RACE 4 OF HETERODFRA GLYCINES

以高抗大豆孢囊线虫４号生理小种的２个大豆品系１２５９系（黄色）和１２５９系（双色）及其抗感亲本灰皮支黑豆和晋遗９号，以及另外两个高抗品种ＰＩ４３７６５４和元钵黑豆，两个高感品种鲁豆１号和鲁豆７号为试验材料，进行大豆抗孢囊线虫的ＰＡＰＤ分析。在所用的３３个引物中，引物ＯＰＧ０４的扩增产物中有一条谱带系抗病品系、抗病品种（包括抗病亲本）特有。

Applicating the randomly amplified polymorphic DNA(RAPD) techniquewe analysedeight soybean cultivars(lines), which included two resistant lines, 1259Y with yellow seed coatand 1259B with bicolor seed coat, and their resistant parent, Huipizhiheidou, susceptible parent,Jinyi 9, other two resistant varieties, Yuanboheidou and P1437654, other two susceptible cultivars, Ludou 1 and Ludou 7, to race 4 of Heterodera glycines for their amplified products.Among33 primers, one primer, OPG04, amplified a DNA fragment which specifically existed in theproduucts of all five resistant varieties and lines, and not found in these of three susceptible cultivars (including susceptible parent).