山羊小腔卵泡卵母细胞的体外成熟和体外受精

Maturation and Fertilization in Vitro of Goat Oocytes Derived from Small Antral Follicles

采集繁殖和非繁殖季节山羊卵巢，回收大腔（３～６ｍｍ）和小腔（＜２ｍｍ）卵泡卵母细胞，进行体外成熟和受精，结果如下：①繁殖季节的大腔和小腔卵泡卵母细胞体外成熟率（６８．４％，２７．５％）均显著（Ｐ＜０．０５）高于非繁殖季节（５２．５％，２０．６％）；②１和１．５周岁龄羊与成年羊相比，前两者的小腔卵泡卵母细胞采集数显著（Ｐ＜０．０５）多于后者（２５．６，２２．５：１５．ｌ），但卵母细胞体外成熟率无显著差异（Ｐ＜０．０５）；③随卵母细胞直径增加，其体外成熟率呈显著上升趋势；④与加新生犊牛血清和生殖激素在颗粒细胞悬浮液中培养相比，加发情山羊血清和激素在颗粒细胞单层上培养可显著（Ｐ＜０．０５）提高小腔卵泡卵母细胞体外成熟率（２０．８％：３４．５％）；⑤将体外成熟的小腔卵泡卵母细胞与体外获能精子进行受精，其受精率为３５．７％（５１／１４３）。

Goat ovaries were collected at breediiig and nonbreeding seasons，The oocytes derived from large（LAF，3-6mm）and small antral follicles（SAF，＜2 mm）were recovered and used for matturation and fertilization in vitro, The results are as follows：①in vitro maturation rate of LAF and SAF oocytes is significantly higher at breeding seasons(68．4％，27.5％)than at nonbreeding seasons（52．5％， 20. 6％）；②the numbers of SAF oocytes collected from the ovaries of goats at l（25．6）and l. 5（22．5）years old is more than that from adult goat ovaries（15．1），but there is no significant difference in oocyte maturation rates among three groups；③in vitro maturation rates of oocytes increases significantly with their diameter increase；④more high maturation rates of SAF oocvtes is obtained when culture in medium with GS，FSH，LH and l7β-E2 on granulosa cell monolayer（34．5％）in comparison with culture in granulosa cell solotion with NCS，FSH，LH and l7β-E2（20．8％）；⑤SAF oocytes maturated were fertilization in vitro with capacited sperm and in vitro fertilization rate is 35．7％ （51／143）.