ELISA法以单一单克隆抗－HBe测定e系统的研究

AN ELISA FOR MEASUREMENT OF HBeAg AND HBeAb

制备了一株ＨＢｅ单抗细胞株，同时用于包被和酶标，以ＥＬＩＳＡ法测定ｅ抗原及病人血清中抗－ＨＢｅ获得成功．

In recent products, sandwich-ELISA and neutralization-ELISA have beenestablished separately for the determination of HBeAg and HBeAb employing two anti-HBemonoclonal antibodies or one monoclonal antibody and one polyclonal antiserum. Monoclonalantibody from one cell clone was purified and used forcoating and peroxidase-conjugating applied in the ELISA Kit for HBeAg and HBeAb. That one monoclonal antibody was availablefor antigenbinding was demonstrated by measurement of HBeAg and human serum of clinicpatients.