小鼠胰岛细胞MHC－DR抗原的诱导表达

Induced-expression of MHC-DR Molecules on Mice Islet Cells

本文报告肿瘤坏死因子（ＴＮＦ）和ｒ－干扰素（ＩＦＮ－ｒ）诱导小鼠胰岛细胞ＭＨＣ－ＤＲ抗原表达对胰岛素分泌的影响。实验结果表明：（１）当ＴＮＦ浓度为５０～１００Ｕ／ｍｌ时未见胰岛细胞表达ＭＨＣ－ＤＲ抗原，而５０Ｕ／ｍｌｒ－ＩＦＮ可诱导少量胰岛细胞（８．５±２．９％）表达ＭＨＣ－ＤＲ抗原；（２）１００Ｕ／ｍｌＴＮＦ和１００Ｕ／ｍｌｒ－ＩＦＮ合并使用，ＭＨＣ－ＤＲ抗原阳性细胞明显增多（８０．２５±４．１１％）；（３）两种因子单独或合并作用均能使体外培养的胰岛细胞的胰岛素分泌量增加（Ｐ＜０．０１）。上述结果提示，胰岛细胞ＭＨＣ－ＤＲ抗原的诱导表达本身并不影响其分泌胰岛素的功能。

The correlation of induced-expression of MHC-DR molecules with insulin release i cul-tured mice islet cells has been studied.The results showed that:(1)No detectable induced-expression of MHC-DR molecules by TNF-a alone at the concentration of 50一100U/ml hasbeen assayed,but the induced-expression of MHC-DR molecules has been identified in a fewislet cells(8.5±2.9%)with IFN-r alone at concentration of-100U/ml.(2)The in-duced-expression of MHC-DR molecules has been detected in as high as 80% of cultured isletcells with TNF-a and IFN-r combined at different concentrations.(3)Insulin released fromcultured islet cells was enhanced by IFN-r and TNF-a either alone or combined at different concentrations.These results suggest that the induced-expression of MHC-DR molecules themselves does not damage the function for insulin release in cultured islet cells.