甲醛-高锰酸钾化学发光体系测定左旋多巴

Determination of Levodopa By the Chemiluminescence System of Potassium Permanganate-Formaldehyde

在酸性介质中,甲醛与高锰酸钾能够产生微弱化学发光,而左旋多巴能增强该发光强度.在一定范围内,其发光强度与左旋多巴的浓度呈良好的线性关系,结合流动注射分析技术,建立了一种测定左旋多巴的新方法.线性范围为1.2×10-8～2.2×10-6g/mL,方法的检出限为4×10-9g/mL.对浓度为6.0×10-7g/mL左旋多巴平行测定11次,其相对标准偏差为1.66%.该法用于左旋多巴片含量测定并与标准方法比较,结果满意.

A new flow injection chemiluminescence（FICL） method for the determination of levodopa was established. This method was based on the enhancement of levodopa on the chemiluminescence reaction of formaldehyde withpotassium permanganate. The concentration range of linear response was 1.2×10^-8-2.4×10^-6 g / mL. The detection limit was 4×10^-9 g / mL with a relative standard deviation of 1.66% for the determination of 6.0×10^-7 g / mL of levodopa（n= 11）. The method was used to determine the content of levodopa in tablets with satisfactory results.