瓦茸多糖体对照射过γ射线小白鼠脾脏损伤之修复作用(英文)

Restorative effect of Coriolus versicolor polysaccharides against r-irradiation-induced spleen injury in mice

目的:评估瓦茸多糖体对于照射过γ-射线小白鼠脾脏损伤之修复作用。方法:本研究所使用之药品为瓦茸多糖体(Coriolus versicolor polysaccharides,CVP,PSK)。瓦茸(又名黑云茸)多糖体是一种与蛋白质结合的多糖体,CVP是从培养的担子菌类胡孙眼科瓦茸之菌丝体精制而成。本研究选取6—8周大之ICR种系小白鼠180只,分为3组:A组为正常对照组;B组为辐射对照组,仅接受1Gyγ射线的全身辐射;C组为实验组,于接受1Gyγ-射线之次日起10d,每日用胃管喂以CVP60mg·kg^(-1)。于辐射后的第5,12,19,26及33日,分5批处理,每批每组各取小鼠8只测量体重后,取出脾脏称重,制成脾脏细胞的悬浮液。用[~3H]TdR追踪法测定脾脏细胞之DNA合成速率。结果:小鼠在接受γ-射线后,其脾脏重量及脾脏细胞之DNA合成速率皆有下降。在照射后接受CVP处理之C组,CVP能促进提早恢复因γ-射线所导致的抑制效果。结论:CVP对于照过γ-射线小白鼠之脾损伤具有显著修复作用。

AIM: To study the restorative effect of Coriolus versicolor polysaccharides (CVP) on spleen injury induced by 7-ray irradiation in mice. METHODS: ICR mice, 6 - 8 wk old, were divided at random into 3 groups: A) normal control; B) irradiated with 1 Gy ; and C) after 1 Gy irradiation, given CVP 60 mg·kg-1(ig) daily for 10 d continuously. Body weight (BW), spleen weight (SW), relative SW (RSW), DNA synthesis of splenocytes (DNA-SS), and relative DNA-SS were measured on d 5, 12, 19, 26, and 33 after irradiation. RESULTS: SW, RSW, DNA-SS, and relative DNA-SS decreased after irradiation. CVP enhanced the recovery of SW, RSW, DNA-SS, and relative DNA-SS inhibited by irradiation. CONCLUSION: CVP has the restorative effect against spleen injury induced by r-ray irradiation in mice.