牛磺酸、硫氮酮单用及合用对大鼠再灌心律失常的预防作用(英文)

Prophylactic effects of taurine and diltiazem alone or combined on reperfusion arrhythmias in rats

目的:研究牛磺酸(70mg·kg^(-1)),硫氮(艹卓)酮(1mg·kg^(-1))合用对麻醉大鼠再灌注心律失常的影响。方法:冠脉结扎15 min后再灌诱发心律失常。硫代巴比妥酸法和分光光度法分别测定MDA和SOD。结果:两药合用的效果优于各药小剂量单用。联合用药后不仅更显著降低缺血/再灌心肌的MDA含量,尚可增加SOD活性。结论:牛磺酸与硫氮(艹卓)酮合用通过抑制脂质过氧化物的产生和防止心肌细胞内“钙超负荷”发挥抗缺血/再灌心律失常作用。

AIM: To study the effects of taurine (Tau) and dil-tiazem (Oil), alone or in combination, on reperfu-sion arrhythmias in anesthetized rats. METHODS: The arrhythmias were produced by coronary artery ligation for 15 min followed by reperfusion. Mal-ondialdehyde (MDA) content and superoxide dis-mutase (SOD) activity were measured by thiobarbi-turic acid fluorescence assay and colorimetric determination. RESULTS: Tau 70 mg·kg-1 in combination with Dil 1 mg · kg -1 were more effective on prevention of the reperfusion arrhythmias than each drug alone. The combination of both drugs not only decreased the content of MDA, but also increased the activity of SOD in reperfusion myocardium. CONCLUSION: The inhibition of lipoperoxides formation as well as the inhibition of the calcium influx was involved in the anti-arrhythmic effect of both Tau and Dil.