人参三醇皂苷对培养心肌细胞的钙通道阻滞作用和抗自由基作用(英文)

Calcium channel blockade and anti-free-radical actions of panaxatriol saponins in cultured myocardiocytes

目的:鉴定人参皂苷R_e,R_f,Rg_1,Rg_2,Rh_1和R_o的钙通道阻滞作用和抗自由基作用。方法:在Wistar大鼠心室肌细胞上,用斑片钳技术记录B、L、T三型单钙通道活动;用ESR法测定自由基含量。结果:R_e,Rg_1,Rg_2,Rh_1使B、L、T三型钙通道的开放时间缩短、关闭时间延长、开放概率减小,R_f使L型钙通道的开放时间缩短、关闭时间延长、开放概率减小,R_o不影响钙通道活动(60μmol·L^(-1))。R_e,Rg_1,Rg_2,Rh_1拮抗Xan0.42mmol·L^(-1)-XOD 5.3 nmol·L^(-1)诱发的自由基含量增多,R_f和R_o无此作用(30μmol·L^(-1))。结论:R_e,Rg_1,Rg_2,Rh_1兼有钙通道阻滞作用和抗自由基作用。R_ff对L型钙通道有阻滞作用。

AIM: To identify the calcium channel blockade and anti-free-radical actions of panaxatriol saponins Re, Rf, Rg1, Rg2, Rh1, and oleanolic acid saponin R0. METHODS: On ventricular myocytes of Wistar rats, single channel activities of B, L, and T type calcium channels were recorded with the cell-attached configuration of patch-clamp technic, and free radical contents were measured with electron spin resenance method. RESULTS: Re, Rg1, Rg2, and Rh1 shortened the open times, prolonged the close times, and reduced the open-state probabilities of B, L, and T type calcium channels: Rf shortened the open time, prolonged the close time and reduced the open-state probability of L type calcium channel; R0 did not influence the activity of calcium channels (60 μmol· L-1). Re, Rg1, Rg2. and Rh1 antagonized the increase of free radical content induced by xanthine 0.42 mmol·L-1-xanthine oxidase 5.3 nmol · L-1 ; R0 and Rf had no effect (30 μmol·L-1). CONCLUSION: Re, Rg1, Rg2, and Rh1 had both the calcium channel blockade and anti-free-radical actions. Rf had blockade action on L type calcium channel.