α,β-受体激动剂及白细胞对再灌注心律失常及室颤影响的正交分析(英文)

Orthogonal analysis of aggravating effects of α- β-agonists and leukocytes on reperfusion-induced arrhythmias and ventricular fibrillation in Langendorff's rat heart

目的:比较白细胞(Leu),α-受体激动(α-Ago)和β-受体激动(β-Ago)三因素对缺血-再灌诱发的心律失常的影响。方法:采用Langendorff离体心脏模型,以降低灌流量继之恢复灌流量(模拟再灌注)诱发心律失常,对三因素进行正交分析。结果:对Leu未耗竭及已耗竭的心脏,再灌注诱发的VF发生率分别为80％和20％,α-及β-Ago明显升高VF发生率。正交分析表明Leu明显提高VF发生率,三因素组合均增加心律失常分数。结论:三因素中Leu是最为重要的促进恶性心律失常发生的因素。

AIM: To study the effects of leukocyte ( Leu), a-agonist (a-Ago), and β-agonist (β-Ago) on the arrhythmias induced by ischemia and reperfusion to determine which of the 3 factors was the most important one in exacerbating arrhythmias. METHODS: Arrhythmias were induced by the reduction and subsequent resumption of perfused flow in Langendorff's perfused rat hearts. Ventricular tachycardia (VT) and ventricular fibrillation (VF) were recorded on ECG, and the results were orthogonally analyzed. RESULTS: When Leu was present, the incidence of VF induced by ischemia-reperfusion was 80 % . The incidence in Leu-depleted hearts was 20 %, a-Ago and β-Ago elevated it to 60 % and 100 %, respectively. The results by orthogonal analysis demonstrated Leu or a-Ago + β-Ago increased VF incidence. With regard to arrhythmias, arrhythmia score was remarkedly increased by all of 3 factors and various combinations except β-Ago + Leu. CONCLUSION: Among these 3 factors, Leu was the most important one in facilitating reperfusion-induced arrhythmias.