双苯氟嗪对大鼠缺血脑皮层诱发电位和氨基酸含量的影响(英文)

Effects of dipfluzine on cortical somatosensory evoked potentials and amino acid contents in ischemic rat brain

目的:测定双苯氟嗪(Dip)对大鼠缺血脑细胞内外氨基酸含量及皮层体感诱发电位(SEP)的影响。方法:在结扎双侧颈总动脉雌性Wistar大鼠,用HPLC法测定脑透析液和脑组织中的氨基酸含量,用电生理技术测定SEP。结果;Dip ip 50 mg·kg^(-1)可防止缺血所致SEP潜伏期的延长及其幅度的过分增大,降低脑透析液中的谷氨酸、天冬氨酸和甘氨酸浓度以及减轻脑组织中谷氨酸、天冬氨酸、甘氨酸、牛磺酸和γ-氨基丁酸的消耗。结论:Dip能够改善脑缺血所致的皮层功能紊乱和脑内兴奋性与抑制性氨基酸释放失调,为其抗缺血性脑损伤作用提供了进一步的实验证据。

AIM: To evaluate the effects of dipfluzine (1-diphenyl-methyl-4-[ 3-( 4-fluorobenzoyl )]-piper-azine, Dip) on the intra- and extra-cellular contents of the amino acids in brain and the cortical somatosensory evoked potentials (SEP) in rats with cerebral ischemia. METHODS: Amino acids in micro-dialysates and brain tissue ho-mogenates in Wistar rats with bilateral carotid artery ligation (BCAL) were measured by HPLC and SEP was measured by the electrophysi-ological technique. RESULTS: Dip 50 mg·kg-1 ip prevented SEP from prolonging of the latency and overactivity of lowered amplitude, markedly lowered the elevation in extracellular level of glu-tamate ( Glu ) , aspartate ( Asp ) , and glycine (Gly) in intracerebral microdialysates, and alleviated the decrement of intracellular contents of Glu, Asp, Gly, taurine (Tau ), and GABA in brain tissue. CONCLUSION: Dip reduced the disturbance of cortical function and the imbalance between excitatory and inhibitory amino acids in ischemic brain, therefore provided a further evidence for its protective effect on ischemic cerebral damage.