人参皂苷Rb_1和Rg_1对大鼠可逆性局灶性脑缺血的影响(英文)

Influences of ginsenosides Rb1 and Rg1 on reversible focal brain ischemia in rats

目的:研究人参皂苷Rb_1和Rg_1对脑缺血和再灌损伤的影响。方法:用可逆性不开颅大鼠大脑中动脉梗塞(MCAO)模型观察人参皂苷Rb_1和Rg_1对梗塞范围(IS),运动障碍(ND)及钙,钾含量的影响。结果:在2-h缺血,Rb_110—40mg·kg^(-1)iv于MCAO前给药减小IS 20％—49％,使ND由5.1减至4.1—2.3,抑制钙积累22％—50％,减少钾丢失18％—37％。MCA再通后给药减小IS12％—35％,使ND由5.2减至4.3—3.3,抑制钙积累10—40,减少钾丢失17％—30％。在永久缺血,Rb_140mg·kg^(-1)iv可减少IS,ND,钙积累和钾丢失。Rg_1对永久和2-h缺血均无效。结论:Rb_1是保护缺血脑的活性成份,对缺血和再灌损伤均有效。

AIM: To study the influences of ginsenosides Rb1 and Rg1 (active components of the total saponins of Panax ginseng ) on the brains against ischemia-reperfusion injury. METHODS: Rat focal cerebral ischemia was induced by reversible middle cerebral artery occlusion (MCAO) without craniectomy. The influences of ginsenoside Rb1 and Rg1 on infarct size (IS ), neurologic deficit (ND) and the contents of calcium and potassium in the infarct were observed. RESULTS: In a 2-h ischemia, Rb1 10 - 40 mg·kg-1 iv 30 min before MCAO decreased IS by 20 %- 49 % and ND score from 5.1 to 4.1 - 2. 3, and inhibited Ca accumulation and K loss by 22 %- 50% and 18-37 %, respectively; Rb1 10 - 40 mg·kg-1 iv immediately after MCA was recanal-ized decreased IS by 12 % - 35 % and ND score from 5. 2 to 4. 3 -3. 3, and inhibited Ca accumulation and K loss by 10 %-40 % and 17 %- 30 % , respectively. In permanent ischemia, Rb1 40 mg·kg-1 iv reduced IS, ND, Ca accumulation and K loss. However, Rg, 40 mg·kg -1 iv did not show effect on both permanent and 2-h MCAO. CONCLUSIONS: Rb1 protected brain from ischemic and reperfusion injuries.