大鼠胚泡植入前期给亲代阿司匹林致移植胚胎的毒性(英文)

Toxicity to transferred rat embryos after aspirin treatment during preimplantation stage in vivo

目的:探索药源性胚泡异常与着床后胚胎毒性和发育缺陷间的关系。方法:大鼠在孕d3 ig阿司匹林(0.25,0.5,和1g·kg^(-1))。孕d 4将胚泡移植于假孕大鼠(与连续5dig氯丙二醇5mg·kg^(-1)大鼠交配获得)子宫内。临产前检查子宫内胚胎。结果:孕d4给药组胚泡异常率增高,着床率低于对照组,试验组的胚胎吸收率(55.2％,69.5％,和85.2％)与畸胎率(3.8％,44.4％,和25％)呈剂量依赖性增高,活胎率降低(44.8％,30.5％,和14.8％),并与胚泡异常率呈相关性。结论:大鼠在胚泡植入前给阿司匹林可导致呈剂量依赖关系的胚胎毒性和畸胎。

AIM: To explore the relationship between drug-induced blastopathies and post-implantation em-bryotoxicity or developmental defects. METHODS : Pregnant rats on d 3 were given intragastri-cally aspirin (0.25, 0.5, and 1 g·kg-1). On d 4, the blastocysts were transferred into the uterine horns of pseudopregnant rats (made by mating with rats which had been given intragastri-cally 3-chloro-l, 2-propanediol 5 mg·kg-1 for 5 d). Uterine contents were examined at term. RESULTS: The frequency of blastocysts with morphological alterations (FBMA) was increased on d 4 of gestation. The implantation rate was lower than that of the controls. A dose-related increase in resorption (55.2 %, 69.5 % , and 85.2 %) and malformation rate (3.8 % , 44.4 %, and 25 %), and decrease in viability rate of fetuses (44. 8 % , 30. 5 %, and 14. 8 %) were observed in test groups with correlations to FBMA. CONCLUTION: Embryotoxicity and fetal malformations were induced by treatment of aspirin before implantation in a dose-dependent manner.