8个禾本科草坪草品种遗传多样性的RAPD分析

Genetic Diversity Analysis on Eight Turfgrass of Poaceae by RAPD

利用RAPD技术,对禾本科4属4种8个品种的草坪草进行遗传多样性分析。15个有效引物用于PCR扩增,共获得RAPD谱带144条带,多态性带占63.2%。8个草坪草品种间的遗传距离在0.0857～0.2928之间,脱壳狗牙根与普通狗牙根间的遗传距离最小,交战2号与爱神特之间的遗传距离最大。用UPGMA和邻接法进行聚类分析,得到2个拓扑结构基本一致的树系图。同一种不同品种间的亲缘关系最近;不同属种间的遗传距离加大,同族不同属的草坪草基本构成一支;4个暖季型草坪草品种构成一个单系类群,但属于冷季型草种的黑麦草(爱神特)单独构成一支,并未与同族的其它3个冷季型草坪草品种(高羊茅)聚在一起。本研究不支持将黑麦草属与羊茅属放在同一族内的分类处理,并建议将高羊茅划分为过渡类型的草坪草。

The genetic diversity of eight turfgrass varieties representing four species in four genera of family Poaceae was analyzed using random amplified polymorphic DNA （RAPD） method. Fifteen 10-meroligonucletide primers were used for DNA ampification, and 144 DNA bands were generated, of which 63.2% is polymorphic loci. The genetic distances are 0.0857-0.2928 among eight turfgrass varieties, the maximum value is between the variety CrossfirelIof Festuca arundinacea and Accent of Lolium perenne, the minimum value is between the two varieties of Unhulled Cynodon dactylon and hulled Cynodon dactylon. The two dendrograms based on genetic distance by UPGMA and Neighbor-joining method are similar topologically. The relationships among different varieties from the same species are very close. The relationships of inter-genera or interspecies are relatively far, although the different genera of the tribe Chlorideae get together into a clade. The four warm-season turfgrass form a clade. The variety Accent of Lolium perenne, a cool-season turfgrass, has a far relationship to other turfgrass and forms a clade. The study didn＇t support to put the genus Lolium and Festuca into the same tribe Poeae and suggested that the turfgrass Festuca arundinacea be treated as an intergrade type of turfgrass.