醒脑启智胶囊对血管性痴呆小鼠脑组织海马细胞p75受体蛋白的影响

Influence of Xingnaoqizhi Capsule on p75 Proteins of Hippocampus Cell in Mice with Vascular Dementia

目的探讨中药醒脑启智胶囊对血管性痴呆小鼠脑组织海马细胞p75受体蛋白的影响。方法120只昆明种小鼠随机分为假手术组、模型组、醒脑液高剂量组、醒脑液低剂量组、银杏叶液对照组、尼莫地平液对照组,每组20只,采用双侧颈总动脉结扎反复脑缺血再灌注的方法制备血管性痴呆小鼠模型。术后15d进行行为学实验和脑组织的病理形态学观察,并用流式细胞术检测各组海马细胞p75受体蛋白的变化。结果①行为学实验显示,模型组小鼠学习成绩与记忆成绩下降,各治疗组小鼠的学习成绩与记忆成绩均有提高(P<0.05或P<0.01);②光镜下病理形态学显示,术后模型组小鼠脑组织海马区呈缺血性病理改变,各治疗组小鼠病变轻于模型组;③流式细胞术检测显示模型组小鼠脑组织海马细胞p75受体蛋白高于假手术组(P<0.01);各用药组与模型组比较,p75受体蛋白降低(P<0.01)。结论醒脑启智胶囊对血管性痴呆小鼠有明显治疗作用,其作用机制之一是调节海马细胞p75受体蛋白的表达。

Objective To investigate the effects of Xingnaoqizhi capsule on p75 proteins of hippocampus cell in experimental mice with vascular dementia. Methods One hundred and twenty Kunming mice were randomly divided into 6 groups： Sham operation group,model group, high dose Xingnaoqizhi Capsule group, low- dose Xingnaoqizhi Capsule group, GinkgoBiloba control group, Nimodipine control group. Each group included 20 mice. The model of vascular dementia mice were duplicated with the operation of cerebral ischemic reperfusion. At the 15th day the morphologic changes of cerebral tissue were observed, and p75 proteins of the hippocampus cells in mice were determined by flow cytometry （FCM） after praxiology experiment. Results ①Results of praxiology experiment showed that the learning and memorizing performance record in model group were drop and that of other groups were improved （P〈0.05 or P 〈 0.01 ）. ②The pathomorphology indicated that hippocampus of mice brain tissue taking up the pathological changes of ischemia. The pathological changes in each medical group was lighter than that in model group. ③ FCM showed that p75 proteins of the hippocampus cells in mice in model group were higher than that in sham- operation group（P〈0.01 ）. At the same time p75 protein in each rnedical group was lower than that in model group（P 〈 0.01 ）. Conclusion Xingnaoqizhi Capsule could regulate p75 proteins of the hippocarnpus cells to treat vascular dementia.