载脂蛋白 B 基因 Xba Ⅰ多态性和胆囊结石病关系研究

Relationship between apolipoprotein B gene Xba Ⅰ polymorphism and gallbladder stone disease

目的研究载脂蛋白 B 基因 XbaⅠ多态性和胆石病关系。方法对75例胆囊结石病人与112例对照者确定 APOB 基因 Xba Ⅰ多态性的基因型,并对其中41例胆囊切除术病人和11例对照者分析胆汁成分。结果胆石组携带 X+/—基因型和 X+等位基因的频率显著高于对照组(P<0.05)。胆石组病人胆汁胆固醇摩尔百分比和胆固醇饱和指数显著高于对照组,而总脂质降低,差异具有统计学意义。携带 X+等位基因的胆石病人胆汁胆固醇摩尔百分比和胆固醇饱和指数高于X—/—纯合子病人,而总脂质降低。结论 X+等位基因可能是胆石病的易感基因之一。

Objective To investigate the relation of apolipoprotein B （APOB） gene Xba Ⅰ pol ymorphism to gallbladder stone disease. Methods The APOB gene Xba Ⅰ polymorphism was deter mined in 76 patient with gallbladder stone disease and 112 healthy subjects. Meanwhile, biliary composition was assayed in 41 patients subjected to cholecystectomy and 11 control patients. Results The distribution of X2＋/- genotype and X＋ allele was significantly higher in the patients with gallbladder stone disease than in the controls （P〈0. 05）. The patients with cholesterol gallstone had statistically significant higher cholesterol molar percentage and cholesterol saturation index in bile but lower total lipids. Cholesterol gallstone patients with X2-/- genotype had higher biliary cholesterol molar percentage and cholesterol saturation index but lower total lipids as compared with the patients with X-/-. Conclusions X＋ allele may be one of the risk genes for cholesterol gallstone disease.