胆固醇结石病人胆囊黏蛋白基因表达差异的研究

Difference in expression of genes in gallbladder wall in patients with cholesterol gallstone disease

目的研究胆固醇结石病人胆囊黏蛋白基因表达差异,以探讨胆石病发生的分子生物学机制。方法对21例胆囊收缩功能良好的胆囊胆固醇结石病人和12例无胆石的对照组,采用RT-PCR 法测定胆囊 MUC1,MUC2,MUC3,MUC4,MUC5AC,MUC5B,MUC6 mRNA 的表达,以GAPDH 作为内参照。结果胆石组胆囊 MUC1,MUC5AC,MUC5B 和 MUC6表达增高(胆石组 vs对照组:MUC1:67.17±14.06vs36.84±10.00,P>0.05;MUC5AC:90.33±10.24vs53.51±9.38,P<0.05;MUC5B:175.19±7.21vs107.54±23.19,P<0.01;MUC6:71.62±14.36vs44.29±12.35,P>0.05)。MUC3表达在两组间差异无统计学意义。本研究在两组间均未检测到 MUC2和 MUC4基因表达。结论胆囊黏蛋白特别是 MUC5表达增加是胆石病的另一个发病因素。

Objective To determine mRNA expression levels of MUCIN genes in the gallbladder among patients with cholesterol gallstone disease （CGSD）. Methods Total RNA was extracted from the gallbladder wall of 21 patients with CGSD and 12 controls without gallstone. All the patients had gallbladder of normal function and none had disorders of the liver, kidneys and endocrine glands. mRNA expression of MUCIN 1, 2, 3, 4, SAC, 5B and 6 was determined by RT-PCR using GAPDH as an endogenous control. Results mRNA expression levels of MUCIN 1, SAC, 5B and 6 were significantly higher in the patients with CGSD than in the controls （P〈0. 05）. There was no significant difference in mRNA expression of MUCIN 3 between the patients with CGSD and controls （P〉 0. 05）. Contrary to our expectation, none of the patients expressed MUCIN 2 and 4. Conclusions Enhanced expression of MUCIN genes, especially MUCIN 5, might be a factor contributing to gallstone disease.