稀土元素对壮观链霉菌生长及其壮观放线菌素产量的影响

Effect of Rare-Earth Elements on the Growth of Streptomyces Spectabilis and the Yield of Actinospectacin

在培养基中加入微量稀土元素，培养壮观链霉菌（Ｓｔｅｐｔｏｍｙｃｅｓｓｐｅｃｔａｂｉｌｉｓ），观察测定对其生长和壮观放线菌素（Ａｃｔｉｎｏｓｐｅｃｔａｃｉｎ）合成的影响．试验表明，１×１０－６和５×１０－６稀土元素明显促进菌落生长，用琼脂块法测定的抑菌圈平均直径明显大于对照．在５×１０－６稀土斜面上生长的孢子，其发芽率比对照孢子发芽率高约１０％．用杯碟法测定摇瓶发酵液效价的结果显示，含１×１０－６和５×１０－６稀土的发酵液，其相对效价高出对照约７～８％．稀土斜面孢子传至第６代时，其生产力没有明显下降，在４℃下保藏５周的稀土斜面孢子的生产力，基本上保持原有水平．

The effects of rare-earth elements on the growth of streptomyces spectabilis and the yield ofactinospectacin were studied by adding trace rare-earth elements in the culture media. Tests showthat the growth,spore formation and germination of streptomyces spectabilis,the actinospectacinsynthesis and yield stability are obviously promoted by aciding 1 × 10-6 and 5×10-6 rare-earth elements.