芥子气致小鼠耳廓皮肤损伤模型的建立

A mouse ear model of sulfur mustard-induced cutaneous injury

目的:构建芥子气所致小鼠耳廓皮肤损伤模型,为研究芥子气皮肤损伤机制及筛选抗毒药物奠定基础。方法:采用小鼠耳廓称重法观察了16,32,64 mg/m l芥子气5μl分别染毒后小鼠耳廓皮肤的水肿程度;通过光镜、电镜观察了32 mg/m l芥子气5μl染毒后小鼠耳廓皮肤病理变化。结果:芥子气所致皮肤水肿具有剂量、时间效应;光镜下可见微疱、炎症、坏死等病理变化,电镜下可见基底膜结构紊乱、断裂等。结论:小鼠耳廓内侧皮肤以32mg/m l芥子气5μl染毒后,可构建出皮肤损伤模型。

Objective ：To establish a mouse ear model of sulfur mustard （HD）-induced cutaneous injury, and lay foundation for analysis of mechanisms of HD induced cutaneous injury and screening of compounds against HD. Methods：The mouse ear was exposed to 5μl of 16 mg/ml,32 mg/ml,64 mg/ml HD in propylene glycol respectively. Mouse ear edema response was determined by weighing method. Histopathologic and ultrastructural changes of the mouse ear exposed to 5μl of 32 mg/ml HD were observed with light microscopy and electron microscopy. Results： HD produced dose- and time-dependent edema; the light microscopic ：changes included epidermal necrosis and epidermal-dermal separation and dermal inflammation, etc. The ultrastructural changes including the disruption of the basement membrane were observed. Conclusion： Exposure to 5μl of 32 mg/ml HD would be optimal to establish the mouse ear model of HD-induced cutaneous injury.