摘要
以翠冠梨茎段为外植体进行组织培养研究,筛选出了较为适合该品种离体繁殖的基本培养基为1/2M S,并通过无芽茎段培养获得了愈伤组织,通过有芽茎段培养获得了无菌苗.
Using the stems of Cuiguan pear as tissue culture materials, we screened out the basic medium which adapts to the tissue culture of Cuiguan pear stems. This mediun is 1/2MS. Moreover, we obtained some fine calli by culturing the stems without bud and gained some aseptic seedlings in the same way.
出处
《中南林学院学报》
CSCD
北大核心
2006年第1期66-68,共3页
Journal of Central South Forestry University
基金
湖南省三项基金项目"湖南沙梨主要栽培品种的自交不亲和性研究"(COOJZY2155)的部分内容
关键词
梨
翠冠
有芽茎段
无芽茎段
组织培养
pear
Cuiguan pear
stems with bud
stems without bud
tissue culture
