铜绿假单胞菌β-内酰胺类抗菌药物耐药相关基因研究

Drug-resistant Genes Associated β-Lactams in Pseudomonas aeruginosa

目的了解临床分离的铜绿假单胞菌中β-内酰胺类抗菌药物耐药相关基因存在状况。方法自临床分离的39株铜绿假单胞菌,采用聚合酶链反应(PCR)检测耐药基因(TEM、SHV、OXA-1群、OXA-10群、PER、VEB、GES、CARB、IMP、VIM、SPM、GIM、OprD、DHA和OprD2)。结果39株PAE中TEM阳性1株(2.6%)、CARB阳性12株(30.8%)、VIM阳性9株(23.0%)、DHA阳性3株(7.7%),39株oprD2基因均为缺失(100.0%),其余基因均阴性。结论临床分离的铜绿假单胞菌VIM及CARB基因携带率高,oprD基因缺失突变是对亚胺培南耐药的重要原因,检出CARB基因为国内首次报道。

OBjECTIVE To investigate the drug-resistant genes associated β-lactams in Pseudornonas aeruginosa isolated in Shaoxing, Zhejiang Province. METHODS Thirty nine strains of P. aeruginosa were isolated from hospitalized patients,and drug-resistant genes were detected by PCR. RESULTS The positive rates of genes of TEM, CARB, VIM and DHA were 2.6%, 30.8%, 23%, and 7.7%, respectively, oprD2 gene was all lack. Genes of SHV, OXA 1 groups, OXA-10 groups, PER, VEB, GES, IMP, SPM and GIM were not found in all 39 isolates tested. CONCLUSIONS There are very high positive percentages of CARB and VIM genes in P. aeruginosa isolated clinically. The oprD2 gene lack is the important mechanism of P. aeruginosa resistant to imipenem. The CARB genes in P. aeruginosa are firsted report domestically.