FGL2基因启动子多态性与HBV感染关联研究

FGL2 Gene Polymorphism among Patients Infected with HBV

目的 探讨FGL2基因启动子（位点-1285T／A）多态性与HBV感染有否关联存在。方法 选择传染科门诊及住院部2002-2004年诊治的重庆地区汉族居民2600人，采用聚合酶链反应（PCR）及限制性内切酶技术检测FGL2基因启动子（-1285T／A）多态性，SPSS软件x^2检验及以非条件Logistic回归进行统计检验。结果 Hardy-Weinberg平衡检验P〉0．05，表明拟合度优良；各组间等位基因频率差异无显著性（P〉0．05）；以非条件Logistic回归校正年龄及性别因素进行分层分析，表明该位点SNP与HBV感染之间无关联存在（P〉0．05）。结论 FGL2基因启动子（一1285T／A）多态性与HBV感染有之间无关联。

OBJECTIVE To characterize the relationship between promoter of FGL2 gene （site-1285T/A） polymorphism and clinical subgroups infected with HBV. METHODS The genotype was analyzed by using method of polymerase chain reaction （PCR）, enzyme restriction after PCR amplification, and agarose gel electrophoresis. Statistics were used Zz and Logistic regression. RESULTS There were no statistic genotype or allele frequency differences between any two subgroups （except acute hepatitis B and liver cancer）. CONCLUSIONS Promoter of fgl2 gene （site-1285T/A） polymorphism has no relation with HBV infection.