ALA口服光动力学治疗鼠G22胶质细胞瘤的研究

Research of ALA-PDT in Treatment of G22 glioma of Rats

目的:通过鼠肿瘤动物模型确定ALA口服激光光动力疗法促使肿细胞死亡及凋亡的疗效及光敏药物最佳用药剂量。方法:以不同剂量的ALA口服(20μg/ml、40μg/ml、60μg/ml、120μg/ml、240μg/ml)、以630nm半导体激光200mW/cm2照射,每光斑照射20分钟剂量,照光前及照光后不同时间以肉眼、肿瘤大小测定、病理、电镜、流式细胞仪观察疗效差别并与未作光动力学治疗的空白对照组模型作比较,寻找最佳的光敏药物配合的剂量。结果:随ALA口服剂量增加PDT疗效增加,口服剂量达60mg/kg疗效明显增加能抑止肿瘤生长,肿瘤变暗,质变硬,结痂,60mg/kg,120mg/kg,240mg/kg疗效相似,并不能达到完全的杀除肿瘤,该法作为肿瘤治疗若与HPD-PDT联合使用,可提高疗效,减少HPD剂量,减少避光时间,ALA-PDT可作肿瘤诊断,可避免了HPD光敏诊断的皮肤光毒反应。结论:光动力学治疗脑瘤口服剂量达60mg/kg疗效明显增加,能抑止肿瘤生长,剂量再增加疗效相似,并不能达到完全的杀灭肿瘤。

Objective： To ascertain the adequate dosage of ALA -PDT which induced tumor cell death or apoptosis on G22 glioma of rats. And to study the different effect of ALA-PDT. Methods： Rat ascitic tumor cells（G22 glioma） were randomly divided into several groups and incubated with ALA take orally（20μg/ml ,40μg/ml, 60μg/ml, 120μg/ml、 240μg/ml）,dosages. 630nm light was delivered to tumor at a constant fluence rate： 200mW/cm^2 and a constant irradiated time period.. 20 minutes. We set 3 groups （no photosensitizers or no irradiation or neither） to be the control groups. We use unaided eye,knubbly size bedetermine,pathology and electron microscope to observe the morphological exchange of tumor and flow cytometry technology to detect the death or apoptosis of tumor cells during the experiment. Results： We use unaided eye,knubbly size bedetermine, pathology and electron microscope to observe the morphological exchange of tumor and flow cytometry technology to detect the death or apoptosis of tumor cells during the experiment. After irradiated with 630nm light, 200mW/cm^2 on 20 minutes, G22 glioma tumor cells incubated with the dosage of ALA60μg/ml were least dosage induced highest rate of apoptosis and putrescence. Conclusiont The dosage of ALA 60μg/ml used in the PDT treatment was the most adequate dosage on in treatment of G22 glioma of rats.