hVEGF基因工程生物膜覆盖大鼠全层皮肤缺损创面的模型建立

Model Establishing of Rat Skin Wounds Covered with hVEGF Genetically Engineering Membrane

目的建立含人血管内皮细胞生长因子(hVEGF)重组质粒的成纤维细胞种植的基因工程生物膜覆盖大鼠全层皮肤缺损创面的动物模型。方法在SD大鼠背部皮肤制作深达皮肤全层的正方形创面,实验组SD大鼠创面上覆含hVEGF重组质粒的成纤维细胞种植的基因工程生物膜,对照组包括3组,分别在大鼠创面上覆整合空质粒的成纤维细胞的生物膜、空白生物膜和仅覆切口膜。采用形态学方法,观察术后3、7、14、29 d实验组和对照组大鼠创面肉芽组织生长及创面愈合情况。结果在各时相实验组大鼠创面中成纤维细胞增生数及新生的毛细血管数均高于对照组大鼠。结论将含hVEGF重组质粒的成纤维细胞种植的基因工程生物膜覆盖于大鼠全层皮肤缺损创面,能诱导血管生成,强有力地促进肉芽组织形成。

Objective To establish a model of rat skin wounds which was covered with the genetically engineering membrane planted by those integrated with hVEGF recombinant plasmid. Method Wounds with full-thickness cutaneous defect were produced on the dorsum of the SD rats. The rats were randomly divided into four groups. For the rats in the expeiimented group, the wounds were covered with hVEGF genetically engineering membrane. For the three control groups, the wounds were covered with membrane planted by the fibroblast integrated with blank plasmid, blank membrane and Tegaderm membrane respectively. The specimens were obtained at the 3,7,14 and 29 days after injury, and examined histopathologically. Results Compared to that of Control groups, the hyperplasia of fibroblast and new Capillary vessel in rat skin wounds were increased at all different time after injury in experiment group. Conclusion Vasculogenesis and granulation tissue growth were accelerated in skin wound which was covered with the genetically engineering membrane planted by those integrated with hVEGF recombinant plasmid.