流动注射化学发光法测定司帕沙星的研究

Chemiluminescence determination of sparfloxacin by flow injection analysis

在酸性介质中，司帕沙星对NaNO2-H2O2发光体系有很强的增强作用，并且其增强化学发光强度与司帕沙星的物质的量浓度在一定范围内呈线性关系。基于此，采用流动注射技术，建立了一种简单、快速测定司帕沙星的方法。线性范围为1．0×10^-7～8．5×10^-4mol/L，检出限为6．5×10^-8mol/L。利用该法测定了司帕沙星片剂中的司帕沙星，回收率在98．8％～104．4％之间。

A flow-injection chemiluminescence （CL） method is described for the determination of Sparfloxacin, based on the enhancement of the CL intensity of sodium nitrite-hydrogen peroxide by sparfloxacin in acidic medium. The method is fast, simple. The linear range is 1.0 × 10^-7 - 8.5 × 10^-4 mol/L. The detection limit （S/N = 3） is 6.5 × 10^-8 mol/L. The proposed method was applied to the determination of Sparfloxacin in tablets with satisfactory results.