牛尿苷酸合酶缺乏症PCR-RFLP检测方法的建立

The Development of PCR-RFLP for Detecting Deficiency of Uridine Monophophate Synthase

提取牛血液和精液DNA,进行PCR扩增、RFLP分析,建立尿苷酸合酶缺乏症(DUMPS)检测方法。对济南市6个奶牛场224头荷斯坦奶牛及53头种公牛进行了DUMPS的PCR-RFLP检测,共检测出2头杂合母牛(携带者),杂合子占检测母牛群的0.89%,在荷斯坦公牛中只检测到一种基因型,没有发现隐性突变基因的携带者。实验证明,该方法重复性好、灵敏性高、稳定性可靠。

The Mothod for detecting DUMPS were developed by extracting DNA from blood and semen,performing PCR and RFLP.Blood samples from 224 Chinese Holstein dairy cattles in 6 herds from Jinan,semen samples from 53 Chinese Hostein bulls were tested to determine whether they are DUMPS-free or DUMPS-carriers,by PCR-RFLP.Two heterozygotes （DUMPS-carrier）were detected from Chinese Holstien dairy cattles.The DUMPS-carriers prevalence in Holstein dairy cattle were estimated to be 0.89%.only wild genotype was found in Chinese Holstein bulls,without the recessive DUMPS gene.The method have a strong repeatability,a high specificity,and good sensitivity.