接骨板诱发局部骨质疏松的细胞机理研究

Cellular Basis of Regional Osteoporosis Induced by Plate Fixation:An Experimental Study on Role of Osteocytes

实验选用健康成年新西兰兔５０只。其中４５只动物在左右侧胫骨分别作不锈钢和有机玻璃接骨板固定，５只作正常对照。术后１ｄ、３ｄ，１周、２周、４周、８周、１２周、１６周、２４周分批处死动物，每批５只。取材分别作光镜、透射电镜和组织学定量分析。结果表明，两种接骨板固定均可使固定骨段骨吸收增加，引起局部骨质疏松。与有机玻璃接骨板固定相比较，不锈钢接骨板固定后骨质疏松发生快，程度重。接骨板固定后的骨吸收包括破骨细胞性骨吸改和骨细胞性骨吸收。接骨板固定后骨细胞的变化可能对破骨细胞性骨吸收起调控作用。

Fifty New Zealand white rabbits were us ed in this experiment. Five animals were served as control and the other 45 were plated on their left tibia with stainless steel plates and right tibia with plastic plates.The plated animals were sacrificed in groups of 5 successively at 1 day,3 days,1 week, 2 weeks,4 weeks,8 weeks,12 weeks,16 weeks,and 24 weeks after operation. The corticcal bone beneath the plates were prepared for light microscopic,transmission electron microscopic observation and quantitative histological analysis. The results indicated that plate fixation did induce the increase of bony resorption causing theregional osteoporosis of plated bone, which occurred more quickly and more severely in stainless plate side than in plastic plate side.The bony resorption included both osteoclastic and osteocytic;The results also showed that the changes of structure and function of osteocytes after plate fixation would infiuence and regulate ahd control osteoclastic resorption.