摘要
从多种真菌中筛选到3株脱氮活性较高的真菌,并将其与一株从武汉市郊新筛选分离到的具有较强脱氮能力的细菌HS-03进行混和培养,通过正交实验确立其最佳培养条件。正交实验方差分析表明:碳源、pH值和温度3个因素对脱氮率的影响都非常显著,菌种因素对脱氮率有一定影响。实验结果表明,真菌与细菌混合脱氮的最佳培养条件为:以琥珀酸钠为碳源,NaNO3为N源,C/N为10,pH=7.5,温度30℃。细菌HS-03和真菌Fusariumoxyspurum的组合优于其它组合。在最佳培养条件下,该组合能在21h内去除10mmolNO3-,优于相同条件下细菌HS-03或真菌F.oxyspurum的反硝化能力。
Denitrification with fungi and bacteria was studied by mixing-culturing three species of fungi and a new bacterium HS-03 that was isolated and selected from somewhere in Wuhan suburbs.Orthogonal experiments were conducted to determine the optimum cultural condition,and the denitrification capability was measured.
出处
《环境科学与技术》
CAS
CSCD
北大核心
2006年第3期9-10,45,共3页
Environmental Science & Technology
基金
国家自然科学基金项目资助(30170011)
关键词
反硝化真菌-细菌
优化组合
正交实验
培养条件
toluene
fungi and bacteria for denitrification
othobonal experiment
cultural condition
