摘要
鸡胚胎干细胞是一种多能性干细胞,从Ⅹ期胚盘分离胚盘细胞或早期鸡胚的生殖嵴分离原始生殖细胞,经体外长期抑制分化培养可得到鸡胚胎干细胞。为维持细胞在培养过程中的未分化状态,需要采用饲养层细胞培养,同时设计合理的培养液配方并添加多种抑制分化或促进增殖的细胞因子。通过碱性磷酸酶活性检测、胚胎表面特异性抗原检测、分化试验及嵌合体试验等方法,可对鸡胚胎干细胞进行准确鉴定。文章主要就鸡胚胎干细胞的分离、培养与鉴定方法的研究进展及其应用前景进行简要综述,为进一步发展更高效的鸡胚胎干细胞培养体系并应用于生产实践提供一定的借鉴。
Chicken embryonic stem cells were pluripotent stem cell which derived from stage blastoderm cells or PGCs isolated from genital ridge by long-time restraining differentiation in vitro. Feeder layer cells and suitable culture medium that supplemented with cytokines of promoting proliferation or restraining differentiation were crucial to keep chicken ES in an undifferentiated state. The chicken ES could be identified by AKP test, SSEAs detection, differentiation experiment and chimera production. The review mainly summarized the isolation, culture in vitro and identification of chicken ES and its application, and it provided some reference for founding more efficient culture system and applying it to production.
出处
《动物医学进展》
CSCD
2006年第1期9-13,共5页
Progress In Veterinary Medicine
基金
广东省自然科学基金资助项目(5012371)
关键词
胚胎干细胞
培养
鉴定
应用
鸡
embryonic stem cell
culture
identification
application
chicken
