猪伪狂犬病PCR诊断方法的建立

The Establishment of PCR Diagnositic Technique of Porcine Pseudorabies

根据伪狂犬病病毒(PRV)gB基因的序列,设计并合成了一对引物,以闽A株细胞培养毒为模板,筛选最佳反应条件,建立了检测PRV的PCR方法,应用本方法对分离的病毒进行基因扩增,获得了217bp的特异性DNA片段,证明了对分离病毒检测的特异性和敏感性,为伪狂犬病的快速诊断提供了条件。

One pair of primers that amplified the g^B gene of Pseudorabies virus（PRV） was designed and synthesized , PCR technique detecting the DNA of PRV was established after selecting the best reaction conditions. This technique was applied to specifically amplify the 217 bp DNA fragment of the PRV. The results showed that the establishment of PRV PCR technique provided a more sensitive, specific and reliable method for pseudorabies diagnosis.