肝硬化、肝细胞肝癌中PTEN的表达及其临床意义

Expression of PTEN in hepatocirrhosis and hepatocellular carcinoma and its clinical significance

目的:探讨PTEN在肝硬化、肝癌组织中的表达及其临床意义。方法:用S-P免疫组织化学方法分别检测71例肝细胞性肝癌(HCC)15例肝硬化及10例正常肝脏PTEN蛋白表达。结果:10例正常肝组织中PTEN蛋白均为阳性表达。HCC中PTEN蛋白的阳性率为69.0%(49/71),明显低于肝硬化组(93.3%)及正常肝组织(100%)(P<0.01)。PTEN蛋白在高分化、中分化、低分化肝细胞性肝癌中的阳性率分别为73.5%、69.6%和57.1%,肝细胞性肝癌中PTEN蛋白阳性表达率与肿瘤的组织分化及其它临床病理因素无关(P>0.05)。肝癌PTEN蛋白阳性表达率与有无合并肝硬化无关(P>0.05)。结论:PTEN表达缺失在肝细胞性肝癌的发生和发展过程中发挥重要作用,检测PTEN蛋白表达有助于肝细胞性肝癌的诊断。

Objective To investigate the expression of PTEN in hepatocirrhosis and hepatocellular carcinoma and its clinical significance. Methods 71 cases of hepatocellular carcinoma （HCC）,15 cases of hepatocirrhosis, 10 cases of normal liver were selected for PTEN protein expression by S-P immunohistochemistry. Results The expression of PTEN encoding product in normal liver was 100%,for HCC the expression rate of PTENprotein was 69.0%,which was obviously lower than the hepatocirrhosis （93.3%） and the normal liver （100%）（P〈0.01 ）.The PTEN protein expression rate in high differentiation,medical differentiation,low differentiation HCC was 73.5% , 69.6%,57.1% respectively. No significant correlation was observed between the PTEN protein expression rate in HCC and tumor differentiation or other clinicopathological manifestations （P〉0.05）.The PTEN protein expression rate in HCC is not related to occurence of hepatocirrhosis （P〉0.05）.Conclusion Negative PTEN expression is one of the important factors to determine the process of the occuring and the developing of HCC,the detection of PTEN protein expression may be useful for the diagnosis of HCC.