尖锐湿疣患者外周血CD14^+单核细胞Toll样受体的表达

Expression of Toll-like receptors on peripheral CD14^+ monocyte in patients with condyloma acuminatum

目的探讨CD14^+单核细胞Toll样受体(TLRs)的表达在尖锐湿疣发病机制中的作用。方法采用双色免疫荧光染色流式细胞术检测了34例尖锐湿疣患者外周血CD14^+单核细胞表面TLR1、TLR2及TLR4的表达。结果尖锐湿疣患者外周血CD14^+单拭细胞TLR4表达水平为(4．8±1．7)％,明显高于对照组的(2．6±1．0)％(P<0．001)；CD14^+单核细胞TLR1的表达水平为(11．8±3．8)％,明显低于对照组的(16．4±5．1)％(P<0．001)；TLR2为(90．8±6．6)％,与对照组的(90．4±6．7)％相比,差异无统计学意义(P>0．05)。结论单核细胞表面的TLR4在对病毒识别、增强特异性的信号传导、启动抗病毒的固有免疫反应中起着重要作用。

Objectlve To study the expression of Toll - like receptors in patients with condyloma acuminatum. Methods Immunoflurescent two - color flow cytometry was used to determine the expression of TLR1 ,TLR2 and TLR4 on CD14^＋ monocytes in 34 patients with condyloma acuminatum and 33 normal controls. Results The expression of TLR4 on CD14^ ＋ monocytes was 4,8 % ±1.7% ,significantly higher than that of controls （2.6% ±1.0% ）（ P 〈 0.001）, Expression of TLR1 was 11,8% ±3,8%）significantly lower than that of controls（ 16.4 % ±5.1% ） （ P 〈 0.001 ）, While the level TLR2 expressed in the patients was 90.8 % ±6.6 % showing no significant difference as compared with that of the controls（90.4% ±6.7 % ）（ P 〉 0.05）, Conclusion TLR4 on monocyte may play an important role in recognition of virus, enhancement of specific signal traasduetion , initiation inherent antiviral immune response in human body.