VERO细胞狂犬病疫苗免疫效果的观察

Observation on the immune results of Vero cell rabies vaccine

目的比较氢氧化铝佐剂和无佐剂Vero细胞狂犬病疫苗免疫效果的影响。方法以常规免疫程序用氢氧化铝佐剂与无佐剂两种疫苗分别免疫豚鼠。并分别于3、7、14、30、60、90、180和360d采血并以小鼠中和法测定不同时间抗体水平。结果氢氧化铝佐剂组和无佐剂组,抗体出现时间不一致,无佐剂组稍早,阳转率于第3d已为5．0％,而佐剂组为0,两组于第60d皆达到100．0％；抗体水平两组也稍有差别,无佐剂组较高,但比较差异无显著性,在第60d达到高峰,第90d逐渐下降。结论初步显示,无佐剂Vero细胞狂犬疫苗免疫效果较佐剂组稍好。

Objective To compare the impact of Vero cell rabies vaccines with aluminium hydroxide adjuvant and that without adjuvant on the immune results in Guinea pigs. Methods The Guinea pigs were immunized with the Vero cell rabies vaccines with and without aluminium hydroxide adjuvant, The antibody levels in mice were determined 3, 7, 14, 30, 60, 90, 180 and 360 days after immunization with neutral test. Results The time of antibody production was different in the groups with and without aluminium hydroxide adjuvant , being a little earlier in the group without aluminium hydroxide adjuvant with positive conversion rate of 5.0% on the third day after immunization and the positive conversion rate in the group with aluminium hydroxide adjuvant was zero. The positive conversion rates reached 100.0% on 60th day after immunization in both groups. The antibody level was higher in the group with aluminium hydroxide adjuvant, but without significant difference. The antibody level was decreased gradually 60days after immunization. Conclusion The immune results of Veto cell rabies vaccine without aluminium hydroxide adjuvant is better that that with aluminium hydroxide adjuvant.