移植人羊膜细胞对大鼠创伤性脑损伤的实验研究

Experimental study on the effects of transplanting human amniotic cells into the rats with traumatic brain injury

目的探究大鼠TBI后脑内移植人羊膜细胞(HACs)对大鼠运动功能的影响。方法 HACs经分离、Hoechst33342标记后重悬调整细胞浓度为105／μl;采用改进的Feeney自由落体法打击大鼠脑皮层后肢运动区域,损伤后24h经微量注射器和立体定向仪将Hoechst33342标记的HACs 10μl分别移植于挫伤灶中心和挫伤灶边缘;在TBI后的28d内采用钉板平衡木行走测试大鼠运动功能变化,运动功能检测结束后取出脑组织行组织学检测。结果治疗组滑落脚步数明显少于对照组 (P<0．05);移植的HACs呈蓝色荧光;部分移植HACs可见MAP-2阳性表达。结论移植HACs使大鼠TBI后运动功能明显改善。

Objective To explore the effects on the motor function after transplanting human amniotic cells （HACs） into cerebrum of rats with traumatic brain injury. Method HACs were resuspended at a concentration of 10^5/μl after HACs were separated and labeled with Hoechst33342. Hindlimb sensorimotor cortex area in rats was impacted by improved Feeney＇s weight drop techniques. 10μl Hoechst33342-labeled HACs were injected into the center and margin of contusion by microsyringe and and stereotactic frame 24h after injury. The motor function was evaluated by pegged beam walking test during 28 days after injury. Then the rats were sacrificed for histological analysis. Results Footslips of the treatment group significantly reduced compared with that of the control group （P 〈 0.05） . Grafted HACs showed blue fluorescence. Some HACs expressed neuron specific antigen-MAP-2. Conclusion The motor deficits of TBI rats was improved by transplanted HACs.