摘要
目的:研究犀角地黄汤对血瘀证血管内皮细胞粘附分子表达的影响。方法:采用免疫组织化学和RT-PCR2种方法观察犀角地黄汤不同剂量(高、中、低)对血瘀证(大鼠)血管内皮细胞胞间粘附分子-1(ICAM-1)、血管细胞粘附分子(VCAM-1)、血小板-内皮细胞粘附分子(PECAM-1)和诱生型一氧化氮合酶(iNOS)表达的影响。结果:模型组ICAM-1、VCAM-1、PECAM-1、iNOS的表达明显高于对照组,犀角地黄汤能减少模型组动物ICAM-1、VCAM-1、PECAM-1、iNOS的表达,而且随着药物剂量的减少,各分子表达递增,呈量效关系。结论:犀角地黄汤能降低血瘀证大鼠血管内皮细胞粘附分子高表达,具有一定的量效关系。
AIM: To study the effects of xijiao dihuang decoction, a Chinese medicine, on the expression of adhesion molecules in vascular endothelial cells (VECs) of adrenine and hypothermia - treated rats. METHODS: The expression of VCAM - 1, ICAM - 1, PECAM - 1, iNOS and their corresponding mRNA in VECs was assayed by immunohistochemistry analysis and RT -PCR, respectively. RESULTS: Immunohistochemistry analysis showed the expression of VCAM- 1, ICAM- 1, PECAM- 1 and iNOS are higher in adrenine and hypothermia - treated rots than that in controls, xijiao dihuang decoction decreased the expression of VCAM - 1, ICAM - 1, PECAM - 1 and iNOS in a dose - dependent manner in adrenine and hypothermia - treated rats. CONCLUSION: Xijiao dihuang decoction can down - regulate the expression of adhesion molecules in vascular endothelial cells of adrenine and hypothermia- treated rats.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2006年第3期547-550,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.30070909)
关键词
肾上腺素
低温
内皮细胞
中草药
粘附分子类
免疫组织化学
Adrenine
Hypothermia
Endothelial cells
Drugs, Chinese Herbal
Adhesion molecules
Immunohistochemistry