犀角地黄汤对肾上腺素与低温处理大鼠血管内皮细胞粘附分子表达的影响

Effects of xijiao dihuang decoction on the expression of adhesion molecules in vascular endothelial cells of adrenine and hypothermia-treated rats

目的:研究犀角地黄汤对血瘀证血管内皮细胞粘附分子表达的影响。方法:采用免疫组织化学和RT-PCR2种方法观察犀角地黄汤不同剂量(高、中、低)对血瘀证(大鼠)血管内皮细胞胞间粘附分子-1(ICAM-1)、血管细胞粘附分子(VCAM-1)、血小板-内皮细胞粘附分子(PECAM-1)和诱生型一氧化氮合酶(iNOS)表达的影响。结果:模型组ICAM-1、VCAM-1、PECAM-1、iNOS的表达明显高于对照组,犀角地黄汤能减少模型组动物ICAM-1、VCAM-1、PECAM-1、iNOS的表达,而且随着药物剂量的减少,各分子表达递增,呈量效关系。结论:犀角地黄汤能降低血瘀证大鼠血管内皮细胞粘附分子高表达,具有一定的量效关系。

AIM： To study the effects of xijiao dihuang decoction, a Chinese medicine, on the expression of adhesion molecules in vascular endothelial cells （VECs） of adrenine and hypothermia - treated rats. METHODS： The expression of VCAM - 1, ICAM - 1, PECAM - 1, iNOS and their corresponding mRNA in VECs was assayed by immunohistochemistry analysis and RT -PCR, respectively. RESULTS： Immunohistochemistry analysis showed the expression of VCAM- 1, ICAM- 1, PECAM- 1 and iNOS are higher in adrenine and hypothermia - treated rots than that in controls, xijiao dihuang decoction decreased the expression of VCAM - 1, ICAM - 1, PECAM - 1 and iNOS in a dose - dependent manner in adrenine and hypothermia - treated rats. CONCLUSION： Xijiao dihuang decoction can down - regulate the expression of adhesion molecules in vascular endothelial cells of adrenine and hypothermia- treated rats.