中药狼疮方对狼疮样BXSB小鼠脾脏免疫系统和淋巴细胞亚群的影响

Effect of lupus recipe on immune system and lymphocyte subsets proliferation in splenic cells of lupus-prone BXSB mice

目的:研究中药狼疮方对狼疮样BXSB小鼠免疫系统和淋巴细胞亚群的影响,探讨狼疮方治疗SLE的免疫学机制。方法:采用BXSB小鼠模型,随机分为3组:狼疮方治疗组(每天0.5mL狼疮方药液灌胃)、强的松治疗组(每天prednisone0.173mg/20gBW溶于0.5mL生理盐水灌胃),未治疗组(每天0.5mL生理盐水灌胃),每组6只,疗程10周。另设与BXSB小鼠同基因的正常C57BL/6小鼠6只为正常对照组。分别采集上述各组小鼠外周血和脾组织进行检测。结果:(1)未治疗组BXSB小鼠血清IgG和抗ds-DNA抗体水平及脾组织CD3、CD4、CD8、CD19、CD23阳性细胞百分比都显著高于正常对照组、狼疮方治疗组、强的松治疗组(P<0.05或P<0.01)。(2)经狼疮方或强的松治疗后,BXSB小鼠血清IgG、抗ds-DNA抗体水平及脾组织CD4、CD8、CD19、CD23阳性细胞百分比显著低于未治疗组(与未治疗组相比,P<0.05或P<0.01),且接近正常水平(与正常对照组比较,P>0.05)。结论:狼疮样BXSB小鼠T、B细胞免疫功能上调。中药狼疮方可抑制狼疮样小鼠T、B淋巴细胞活化,减轻其高丙种球蛋白血症,减少体内自身抗体产生。

AIM： To investigate the effects of lupus recipe on immune system and lymphocyte subsets proliferation in splenic cells in BXSB mice. METHODS： Eighteen male BXSB mice model was used in the experiment. The model mice were divided into three groups： un-treated model group, lupus recipe （LR） treated group, and prednisone treated group. All model mice were killed in 10 weeks. The control group consisted of 6 syngeneic normal C57BL/6 male mice. The levels of total IgG and anti - dsDNA antibody in serum were detected by ELISA. The percentages of lymphocyte subsets （ CD3^＋ , CD4^＋ , CD8^＋ T lymphocytes and CD19^＋ , CD23^＋ B lymphecytes） were detected by using flow cytometry analysis. RESULTS： （1） The serum levels of total IgG and anti - dsDNA antibody in un- treated model group were higher than that in other groups. There was no differences among LR treated group, prednisone treated group and control group. （2） The percentages of CD3^＋ , CD4^＋ , CD8^＋ T lymphocytes and CD19^＋ , Cd23^＋ B lymphocytes in model group were obviously higher than that in normal control. （3） Compared to un- treated model group, the percentages of CD4^＋ , CD8^＋ T lymphecytes and CD19^＋ , CD23^＋ B lymphecytes in LR or prednisone treated group were significantly reduced, which closely reached the levels in normal group. CONCLUSIONS： The immune functions of T and B lymphocytes in BXSB mice are up - regulated. LR inhibits the activation of T and B lymphocytes, reduces the serum levels of IgG and auto- antibody production.