雏鸭胰脏组织损伤在新型鸭肝炎病毒感染过程中的变化特点

Characteristics of the Pancreas Organ Damage in Ducklings Infected with New Type Duck Hepatitis Virus

对新型鸭肝炎病毒人工感染9日龄樱桃谷雏鸭的胰脏损伤特点进行了研究。对感染雏鸭在接毒后12、24、48、72、96、168h以及14d时胰脏病理组织学变化的观察结果表明：感染雏鸭的胰脏组织在接毒后24h出现胰腺细胞的局灶性坏死及嗜酸性小体，且在接毒后48h分布广泛而严重；接毒后72～168h期间，胰脏组织中出现炎性细胞浸润并且逐渐增多，而胰腺的局灶性坏死及嗜酸性小体逐渐减少；接毒后14d，仅见到组织炎性细胞的浸润。应用透射电镜对接毒后48h胰脏的超微结构观察结果显示，胰腺细胞发生坏死．同时出现凋亡细胞的形态特征。本试验结果表明，在新型鸭肝炎病毒感染雏鸭时，胰脏的局灶性坏死是典型病理变化之一，胰腺细胞在发生坏死的同时，可能也发生凋亡，二者同时出现在同一胰脏组织内。

9-day old ducklings were inoculated with new type duck hepatitis virus. The histopathologieal changes of pancreas of inoculated duckling were examined at 12,24,48,72,96,168 h and 14 d post inoculation. Histopathological examination revealed there was local coagulative necrosis and Councilman＇s bodies in pancreas at 24 h post inoculation and it became the most extensive and serious at 48 h post inoculation,between 72 h to 168 h post inoculation,there was heterophil infiltration in pancreas and became more serious. The local coagulative necrosis and Councilman＇s bodies decreased,only heterophil infiltration was observed at 14 d post inoculation. Uhrastructural study revealed the necrosis and apoptosis in pancreas cells. The study implied that the duckling infected with new type duck hepatitis virus resulted in both the necrosis and apoptosis in the pancreas, and the local coagulative necrosis in pancreas was one of the typical histopathological changes.