Fhit蛋白与喉咽癌生物学行为相关性研究

The relationship between expression of Fhit and biological behaviors of hypopharyngeal carcinoma

目的:探讨脆性组氨酸三联体(FHIT)基因蛋白Fhit在喉咽癌组织中的表达状况及与该肿瘤生物学行为的可能关系。方法:采用兔抗人Fhit抗体与枸橼酸-微波-SP免疫组织化学方法检测66例喉咽癌标本中Fhit的表达状况,并分析其与组织学分级、淋巴结转移的关系。结果:①喉咽癌组织Fhit表达阳性率为42.42%(28/66),正常喉咽组织为83.33%(55/66),二者比较,P<0.01;②Fhit在高、中、低分化鳞状细胞癌中的表达阳性率分别为65.38%(17/26)、38.10%(8/21)和26.32%(5/19),各个病理分级之间比较,P<0.01;③无淋巴结转移组Fhit表达阳性率为75.00%(12/16),淋巴结转移组为36.00%(18/50),P<0.01。结论:喉咽癌Fhit的表达较正常组织降低,其表达状况与病理分级及淋巴结转移明显相关,提示Fhit表达降低可能对喉咽癌的演化和进展具有重要作用,并有可能成为一个新的预后指标。

Objective：To investigate the expression of fragile histidine triad gene protein（Fhit） and the possible relationship between Fhit and biological behaviors of hypopharyngeal carcinoma. Method： The expression of Fhit protein in 66 cases with hypopharyngeal carcinoma were detected by rabbit antibody to human FHIT gene protein with SP immunohistochemical method. The relationship between historical grade and lymphatic metastasis were analysed. Result ： ① The positive expression rate of Fhit was 42.42% （28/66） in hypopharyngeal carcinoma, and 83.33% （55/66） in normal hypopharyngeal tissue, respectively. There was a significant difference in the expression of Fhit between the cancer tissue and normal tissue（ P〈0.01）. ②The positive expression of Fhit in well, moderate, poor differentiated hypopharyngeal carcinoma were 65.38% （ 17/26）, 38. 10% （8/21） and 26. 32% （5/19）, respectively. There are significant statistic difference among them each other （ P〈0. 01 ）. ③ The positive expression of Fhit was 75.00%（12/16） in the group without lymph node metastasis, and 36. 00% （18/50） with lymph node metastasis. Conclusion： The expression of Fhit in hypopharyngeal squamous cell carcinoma lower than normal tissue. The expression of Fhit significantly associated with historical grade and lymphatic metastasis in hypopharyngeal squamous cell carcinoma. It suggested that the expression of Fhit decreased may plays an important role in the development and progression of the tumor, and thus may become a new prognostic marker in hypopharyngeal carcinoma.