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糖尿病大鼠冠状动脉超氧阴离子生成部位的研究

The studies on location of producing superoxide anion in coronary arteries of diabetic rats
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摘要 目的探讨糖尿病大鼠高血糖状态下冠状动脉超氧阴离子(O2-)生成部位。方法用链脲佐菌素制备大鼠糖尿病模型后取冠状动脉和在高糖下培养大鼠冠状动脉24 h,免疫荧光组织化学染色及激光扫描共聚焦显微镜检测冠状动脉中O2-生成部位。结果糖尿病大鼠冠状动脉内皮组织、外膜组织和平滑肌组织O1-生成量均较对照组升高,二者比较差异有统计学意义(t内=24.719,P<0.01;t外=14.206,P<0.01;t平:11.784, P<0.01)。高糖培养24 h,大鼠冠状动脉内皮组织O2-生成量比对照组升高,二者比较差异有统计学意义(t内= 2.183,P<0.05);外膜组织和平滑肌组织O2-生成量与对照组比较,差异无统计学意义(t外=1.439,P>0.05;t平= 1.662,P>0.05)。结论糖尿病短期高血糖状态下,O2-只产生于内皮细胞,长期高血糖状态下,O2-由内皮细胞经跨细胞转移转运到平滑肌细胞。 Objective To discuss the producing location of coronary arteries superoxide anion under hyperglycemia status. Methods Rat diabetic model was established by intraperitoneal injection of streptozotocin; rats coronary arteries were cultured in DMEM (high glucose) for 24 h. After immunofluorescent histochemical staining, the coronary arteries were observed under confocal microscopy as for where superoxide anion was rpoduced. Results The average superoxide anion densities in endothelium (e), adventitia (a) and smooth (s) muscle of coronary artery in diabetes model group respectively increased compared with that in control group (te = 24.719, P 〈 0.01; ta = 14.206, P 〈 0.01; ts = 11.784, P 〈 0.01, respectively) ;The average superoxide anion density in endothelium of coronary artery cultured in high glucose for 24 h was higher than that in control group (te = 2.183, P 〈 0.05), but that of adventitia and smooth muscle made no difference(ta = 1.439, P〉 0.05; ts = 1.662, P〉 0.05, respectively). Conclusions Superoxide anion is produced in coronary artery endothelium only under hyperglycemia in a short period of time;but after a long period of time,due to cell membrane transportation function, superoxide anion may be transported from endothelium to smooth muscle across cell membrane.
出处 《中国地方病学杂志》 CAS CSCD 北大核心 2006年第2期160-163,共4页 Chinese Jouranl of Endemiology
基金 国家自然科学基金资助项目(30371647)
关键词 糖尿病 冠状动脉 超氧阴离子 Diabetes Coronary arteries Superoxides
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参考文献7

  • 1Liu Y,Terata K,Rusch NJ,et al.High glucose impairs voltagegated K+ channel current in rat small coronary arteries[J].Circ Res,2001,89(2):146-152.
  • 2周家国,朱邦豪,关永源,贺华,庞瑞萍,林默君.蛋白酪氨酸激酶与糖尿病大鼠主动脉平滑肌高反应性的关系[J].中国药理学通报,2001,17(5):511-514. 被引量:4
  • 3Ungvari Z,Csiszar A,John G,et al.Increased superoxide production in coronary arteries in hyperhomocysteinemia:role of tumor necrosis factor-alpha,NAD(P)H oxidase,and inducible nitric oxide synthase [J].Arterioscler Thromb Vasc Biol,2003,23(3) :418-424.
  • 4Storch WB,阮幼冰,武忠弼,等.免疫荧光基础实验新原理及其临床新应用[M].北京:人民卫生出版社,1999.43-44.
  • 5Maritim AC,Sanders RA,Watkins JB.Diabetes,oxidative stress,and antioxidants:A review[J].J Biochem Mol Toxicol,2003,17(1):24-38.
  • 6Kyselova P,Zourek M ,Rusavy Z,et al.Hyperinsulinemia and oxidative stress [J].Physiol Res ,2002,51 (6):591-595.
  • 7金钟一,赵进军,毕亚艳,尹雪松.联合STZ和ALX制备大鼠糖尿病模型的心肌病理学观察[J].哈尔滨医科大学学报,2005,39(1):58-60. 被引量:1

二级参考文献10

  • 1Cryer PE.We are what we repeatedly do[J] .Diabetes, 1998,47(2):155-158.
  • 2van Hoeven KH, Factor SM. Diabetic heart disease: The clinical and pathological spectrum--Part Ⅰ [J]. Clin Cardiol, 1989, 12 (10): 600-604.
  • 3van Hoeven KH,Factor SM. Diabetic heart disease-PartⅡ: The clinical and pathological spectrum[ J]. Clin Cardiol, 1989, 12( 11 ):667-671.
  • 4Mordes JP, Rossini AA. Animal models of diabetes[J]. Am J Med,1981,70(2): 353-360.
  • 5Sasaki K, Kuriyama H, Yeh LI, et al. Studies on diabetic cataract in rats induced by streptozotocin. I. Photodocumentation of lens opacificafion[J]. Ophthalmic Res, 1983,15(4): 185-190.
  • 6Bailes BK. Diabetes mellitus and its chronic complications [ J ]. AORN J,2002,76(2) :266-276.
  • 7Zhu B H,Life Sci,1999年,65卷,15期,1553页
  • 8Liu C Y,Am J Physiol,1996年,270卷,6 Pt 1期,C1825页
  • 9张均田.现代药理实验方法[M].北京:北京医科大学中国协和医科大学联合出版社,1998.1151.
  • 10郑里翔.STZ和alloxan联合使用复制实验性糖尿病大鼠模型[J].中国病理生理杂志,1999,15(8):758-759. 被引量:16

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