DHT、IL-6和IL-8对卵巢癌细胞体外增殖的作用

Impact of DHT, IL-6 and IL-8 on proliferation of epithelial ovarian carcinoma cells in vitro

目的探讨雄激素与细胞因子在上皮性卵巢癌生长中可能存在的相互调节作用。方法应用免疫印迹（Western blot）和RT-PCR技术对5种常见的上皮性卵巢癌细胞系雄激素受体（AR）、ID6受体（IL-6Rα、gp130）及IL-8受体（IL-8RA、IL-8RB）的表达进行检测，选择兼有AB、IL-6R及IL-8R的卵巢癌细胞系作为研究模型，应用MTT法观察5α-二氢睾酮（5α-dihydrotestosterone，DHT）及IL-6、ID-8两种细胞因子对卵巢癌细胞体外增殖作用的影响。结果（1）5种上皮性卵巢癌细胞系中AR、ID-6Rα、gp130、IL-8RA以及IL-8RB的表达存在差异性。（2）在SKOV-3细胞，低剂量DHT（0．1～1nmol／L）作用后的前72h抑制细胞增殖，中高剂量DHT（1～100nmol／L）作用后的72、96、120h促进细胞增殖；在OVCAR-3细胞，与对照组相比，DHT作用48h时细胞增殖差异无统计学意义，作用96h和144h时才有明显的促增殖作用。DHT对这两种细胞增殖的作用具有明显的剂量依赖性和时间依赖性。（3）AR阻断剂氟他胺（flutamide，Flu）可完全阻断DHT的促增殖作用，而抗IL-6中和抗体和抗IL-8中和抗体则可部分阻断其促增殖作用。（4）ID6和ID8可促进SKOV-3和OVCAR-3细胞增殖，其促增殖作用亦具有一定的剂量依赖性和时间依赖性，且二者在SKOV-3细胞有一定的协同效应，而在OVCAR-3细胞则未见到。ID-6和ID-8诱导的促增殖作用可被其相应的中和抗体完全阻断，而不能被无关抗体即同种型羊IgG所阻断。结论雄激素促进上皮性卵巢癌生长的作用机制可能有二：一是雄激素的直接刺激作用，二是通过调节细胞因子（如ID-6和IL-8）分泌和/或其受体表达量，从而促进细胞的生长。

Objective To investigate the reciprocal regulation of androgen and cytokines in the growth of epithelial ovarian careinoms. Methods The expression of androgen receptor （AR）, interleukin 6 receptor （IL- 6R） and intedeukin 8 receptor （IL-8R） in five epithelial ovarian carcinoma cell lines was detected by Western blot and reverse transefiption polymerase chain reaction （RT-PCR）. Ovarian carcinoma cells expressing AR and IL-6R and IL-8R were selected for research, MTT assay was performed to investigate the effect of 5α-dihydrotestosterone （DHT）, IL-6 and ID8 on the proliferation of the above cells. Results （1） The expression of AR, IL-6Rα,gp130, IL-8RA and IL-8RB in five epithelial ovarian carcinoma cell lines was different. （2） In SKOV-3 cells, a low dose of DHT （0.1-1nmol/L） inhibited cell proliferation, whereas a moderate- and high- dose of that （ 1-100 nmol/L） obviously promoted at 72, 96 and 120 h. For OVCAR-3 cells, DHT had no effect on cell proliferation at 48 h, while DHT markedly enhanced cell proliferation at 96 h and 144 h. The effect of DHT on two cells was in a and dose-dependent manner. （3） Flutamide （Flu）, an AR inhibitor completely blocked DHT-enhanced cell proliferation, hut ID6-neutralizing antibody and IL-8-neutralizing antibody partially blocked DHT-stimulated cell proliferation. （4） IL-6 and IL-8 significantly promoted SKOV-3 and OVCAR-3 cell proliferation in a time- and dose-dependent manner, and both have a synergistic effect in SKOV-3 cells but not in OVCAR-3 cells. IL-6 and IL-8-promoted cell proliferation was completely blocked by specific neutralizing antibodies but not by isotype goat IgG. Conclusion The mechnism of androgen-induced ovarian carcinoma cell proliferation appears to be resulted from the direct action of androgen and the indirect regulation of eytokines （such as IL-6 and IL-8） and/or their receptors