丹酚酸B及丹参酮ⅡA对家兔动脉粥样硬化IL-8及VCAM-1的影响

Effect of salvianolic acid B and tanshinone ⅡA on the expression of the level of interleukin-8 and vascular cell adhesion molecule-1 in atherosclerosis rabbits models

目的:观察丹参水溶性成分丹酚酸B和脂溶性成分丹参酮ⅡA对家兔动脉粥样硬化形成时炎症反应的影响,以探讨其抗动脉粥样硬化的作用靶点与机制。方法:建立家兔动脉粥样硬化模型,放射性免疫方法检测血清白介素-8(IL-8)浓度、免疫组化方法进行主动脉血管细胞黏附分子-1(VCAM-1)染色,观察丹酚酸B和丹参酮ⅡA对家兔动脉粥样硬化的影响。结果:丹酚酸B和丹参酮ⅡA均能减少动脉粥样硬化家兔主动脉VCAM-1的表达,而丹酚酸B作用更明显;丹参酮ⅡA能显著降低动脉粥样硬化家兔血清IL-8浓度,丹酚酸B对IL-8没有明显影响。结论:丹酚酸B与丹参酮ⅡA均可防治动脉粥样硬化,但作用靶点及机制有所不同。

Objective： To explore effect of water-soluble salvianolic acid B and fat-soluble tanshinone Ⅱ A on the reaction of inflammation in atherosclerosis rabbits. Methods. Atherosclerosis models were initiated in rabbits by feeding cholesterol and oil. Effect of salvianolic acid B and tanshinone Ⅱ A on sera IL-8 and VCAM-1 in aorta were observed by radio immunity and immunohistochemistry. Results： Both the salvianolic acid B and .the tanshinone Ⅱ A could markedly decrease the expression of VCAM- 1, but the salvianolic acid B is better than the tanshinone Ⅱ A, and the tanshinone Ⅱ A can significantly decrease the level of IL-8, but the salvianolic acid B has no effect on IL-8. Conclusion： Salvianolic acid B and tanshinone Ⅱ A could inhabit atherogenesis of hyperlipid-fed rabbits, but the target and the mechanism has rome difference.