系统性红斑狼疮患者CD4^+CD25^+调节性T细胞的变化

Change of CD4^+CD25^+ T regulatory cells in systemic lupus erythematosus

目的检测系统性红斑狼疮(SLE)患者外周血CD4+CD25+T细胞和CD4+CD25highT细胞占CD4+T细胞的比率,探讨其在SLE发病中的意义。方法采用流式细胞术检测93例SLE患者及47 名正常对照外周血CD4+CD25+调节性T细胞和CD4+CD25highT细胞占CD4+T细胞的比率,分析其与SLE 临床及实验室指标的关系。结果活动性SLE患者CD4+CD25+调节性T细胞及CD4+CD25highT细胞比例较正常人降低;CD4+CD25highT细胞水平与系统性红斑狼疮疾病活动指数(SLEDAI)呈负相关;CD4+ CD25+调节性T细胞与抗核抗原抗体(ANA)、抗可提取的核抗原(ENA)抗体、抗dsDNA抗体、免疫球蛋白、补体C3、C4无相关。结论CD4+CD25+T细胞和CD4+CD25highT细胞可能参与SLE的发病机制。

Objective To investigate the levels of CD4^＋CD25^＋T regulatory cells and CD4^＋CD25^highT regulatory cells in the peripheral blood of systemic lupus erythematosus （SLE） and its significance in the pathogenesis of SLE. Methods Ninety-three SLE patients and 47 healthy individuals were studied. Levels of CD4^＋CD25^＋T regulatory cells and CD4^＋CD25^high T regulatory cells in the peripheral blood were measured by flow cytometry. Disease activity was assessed by systemic lupus erythemattousus activity index （SLEDAI）. Results The levels of CD4^＋CD25^＋T regulatory cells and CD4^＋CD25^highT regulatory cells in active SLE were significantly lower than normal controls. Furthermore, the levels of CD4^＋CD25^highT regulatory cells in SLE were negatively correlated with SLEDAI. No significant correlation was found between levels of CD4^＋CD25^＋T regulatory cells and other SLE serological parameters including ANA, anti-ENA antibodies, anti-dsDNA, immunoglobulin, complement C3 and C4. Conclusion These data suggest that CD4^＋CD25^＋T regulatory cells and CD4^＋CD25^highT regulatory cells play a role in the pathogenesis of SLE.