聚维酮联合阿霉素对E-J膀胱癌细胞的影响

目的通过聚维酮(PVP)联合阿霉素(adriamycin,ADM)对E-J膀胱癌细胞的影响,为开辟膀胱肿瘤术后膀胱灌注的新方法奠定基础。方法E-J膀胱癌细胞体外培养,实验分3组PVP组(1%PVP)、ADM组、PVP+ADM组,药物与细胞培养12h后用生理盐水冲洗再分别培养24h,流式细胞仪检测细胞凋亡的百分数。结果3组细胞在与药物培养12h后细胞凋亡的百分数分别为2.3%、37.3%、56.7%,生理盐水冲洗再分别培养24h,3组细胞凋亡的百分数分别为3.0%、39.4%、76.4%。结论PVP在体外显著提高ADM抗E-J膀胱癌细胞效应,其作用机理可能在于PVP的粘附性。

Objective To investigate The effect of combiniag polyvidane（PVP） and adriamycin on E - J cells of bladder carcinoma ,thus providing some evidence for applying bladder irrigation therapy after operatian of bladder carcinoma . Methods cell culture of E - J bladder carcinoma cells , they were divided into three groups and were treated with PVP （ 1% PVP）, ADM. PVP ＋ ADM separately , 12h after the administration of the drug to the cell culture , cells were washed with and cultured for another 24h , percentage of cell necrosis was assessed with flow cytometry . Re,tilts Percentage of necrosis of the three groups of cells after 12h cultured with drugs was 2.3% ,37,3% ,56.7%, percentage of cells necrosis in cells cultured for 24h after washed with NS were 3.0%,39.4%,76.4%. Conclusion PVP could significandy increase the ADM＇s anti - cancer effect towards E- J bladder carcinoma cells, the mechanism might be the adhesion character of PVP.