桑寄生对培养的人HepG2细胞葡萄糖消耗作用的影响

Effects of Extract of Herba Taxilli on Glucose Consumption in Cultured HepG2 Cells of Human

目的:研究桑寄生对培养人HepG2细胞的葡萄糖消耗作用,初步探讨桑寄生的降糖作用机理。方法:采用人的培养的HepG2细胞,检测24h后培养液中葡萄糖的消耗量,用MTT法监测细胞增殖的情况。结果:桑寄生在高糖状态下可使HepG2细胞的葡萄糖消耗量增加,对胰岛素刺激的HepG2细胞的葡萄糖消耗量增加有协同作用。结论:促进外周组织的葡萄糖代谢、提高肝细胞对胰岛素的敏感性可能是桑寄生防治2型糖尿病的作用机理之一。

Objective： To observe the effect of extracts of Hcrba taxilli on glucose consumption in cultured HepG2 cells and investigate the hypoglycemic mechanism of it initially. Methods： Cultured HepG2 cells of human were used to detect the 24 hours＂ consumption of glucose in Cultured fluid,MTT assay was used to monitor the proliferation of the HepG2 cells. Results： Herba taxiUi could increase the glucose consumption of HepG2 cells and enhance the glucose consumption in coordination with insulin. Conclusion ： Promoting the glucose consumption rate of peripheral tissue and enhance the sensitivity of hepatocytes to insulin may be one of the possible hypoglycemic mechanisms of Herba taxilli.